INHIBITORY EFFECT OF COVALENT POLYMYXIN B CONJUGATE WITH HUMAN SERUM ALBUMIN ON ENDOTOXIN INDUCED NITRIC OXIDE

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NAJAR PIRAYEH SH.; BEHZADIANNEZHAD GH.; MOAZENI M.; KAZEMNEJAD ANOUSHIRAVAN

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Journal: Pathobiology Research Year:2002 | Volume:4 | Issue:2 Page(s): 189-196

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Title

INHIBITORY EFFECT OF COVALENT POLYMYXIN B CONJUGATE WITH HUMAN SERUM ALBUMIN ON ENDOTOXIN INDUCED NITRIC OXIDE

Author(s)

NAJAR PIRAYEH SH. | BEHZADIANNEZHAD GH. | MOAZENI M. | KAZEMNEJAD ANOUSHIRAVAN | Issue Writer Certificate

Keywords

ENDOTOXINQ3

POLYMYXIN BQ3

ALBUMINQ4

SEPSISQ3

NITRIC OXIDEQ3

Abstract

The ENDOTOXIN from gram-negative bacteria consists of a molecule lipopolysaccharide (LPS), which can be shed by bacteria during antibiotic therapy. A resulting syndrome, endotoxic shock, is a leading cause of multiple organ failure and death. We describe here covalent PMB-HSA conjugate, which has both antiendotoxic and antibacterial activities in vitro. Conjugate of PMB with HSA were prepared by EDAC [1-ethy1-3-(3-dimethylaminopropy1) carbodiimide]. Double Immunodiffusion agar test showed that the purified cojugate contained bound PMB. The PMB Bound in the cojugate retained its ENDOTOXIN-neutralizing activity compared to that of the unbound PMB as evidenced by its dose-dependent inhibition of NITRIC OXIDE (NO) release from murine Macrophages, which was stimulated by Purified E.coli 055:B5 LPS or 106 CFU/ml P. aeruginosa PAO1 filterated.The PMB-HSA conjugate was also bactericidal against E.coli and P. aeruginosa PAO1 with MICs of 400 and 200 microgramlml respectively.These experiments demonstrated that the PMB-HSA conjugate retains bound yet functional PMB and may be useful for the design of drugs for the treatment of gram-negative bacterial SEPSIS.

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APA: Copy

NAJAR PIRAYEH, SH., BEHZADIANNEZHAD, GH., MOAZENI, M., & KAZEMNEJAD, ANOUSHIRAVAN. (2002). INHIBITORY EFFECT OF COVALENT POLYMYXIN B CONJUGATE WITH HUMAN SERUM ALBUMIN ON ENDOTOXIN INDUCED NITRIC OXIDE. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 4(2), 189-196. SID. https://sid.ir/paper/81330/en

Vancouver: Copy

NAJAR PIRAYEH SH., BEHZADIANNEZHAD GH., MOAZENI M., KAZEMNEJAD ANOUSHIRAVAN. INHIBITORY EFFECT OF COVALENT POLYMYXIN B CONJUGATE WITH HUMAN SERUM ALBUMIN ON ENDOTOXIN INDUCED NITRIC OXIDE. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2002;4(2):189-196. Available from: https://sid.ir/paper/81330/en

IEEE: Copy

SH. NAJAR PIRAYEH, GH. BEHZADIANNEZHAD, M. MOAZENI, and ANOUSHIRAVAN KAZEMNEJAD, “INHIBITORY EFFECT OF COVALENT POLYMYXIN B CONJUGATE WITH HUMAN SERUM ALBUMIN ON ENDOTOXIN INDUCED NITRIC OXIDE,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 4, no. 2, pp. 189–196, 2002, [Online]. Available: https://sid.ir/paper/81330/en

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