EFFECT OF DEMECOLICNE TREATMENT ON THE DEVELOPMENTAL COMPETENCY OF IN VITRO MATURED BOVINE OOCYTES

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KHOUB BAKHT Z.; HAJIAN MAHDI; HOSSEINI SEYED MORTEZA; FOROUZANFAR MOHSEN; MOULAVI FARIBA; ABEDI PARVANEH; HOSSEINI LALEH; KARAMI SHABANKAREH H.; NASRESFAHANI MOHAMMAD HOSSEIN

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Journal: Anatomical Sciences Journal Year:2008 | Volume:6 | Issue:24 Page(s): 447-457

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Title

EFFECT OF DEMECOLICNE TREATMENT ON THE DEVELOPMENTAL COMPETENCY OF IN VITRO MATURED BOVINE OOCYTES

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Keywords

DEMECOLICNEQ3

BOVINE OOCYTEQ3

DEVELOPMENTAL COMPETENCYQ3

VIABILITYQ3

Abstract

Purpose: The aim of this study was to investigate whether DEMECOLICNE treatment of matured BOVINE OOCYTEs adversely affects the process of in vitro fertilization and embryo development.Materials and Methods: Bovine Cumulus Oocyte Complexes (COC's) were matured in vitro and then were randomly allocated to two treatment groups of common concentrations of DEMECOLICNE (0.05 and 0.4 mg/ml for 30 min) and a control group. COC's were then fertilized and cultured in vitro for up to 9 days when the ratios of in vitro embryo development and the VIABILITY of the hatched blastocysts were assessed and compared with the control group (p<0.05).Results: The ratios of the cleavage and blastocyst formation of DEMECOLICNE treated groups (0.4 and 0.05 mg/ml) were 68.6, 63.5% and 23.3, 32.8%, which were not significantly different from the control group (73.3, 29.0%), respectively. The results of cell-viability were also not significantly different between the control vs. treatment groups.Conclusion: Since the overall indices of in vitro embryo development revealed no significant difference between the DEMECOLICNE treated compared to control BOVINE OOCYTEs, it seems that DEMECOLICNE treatment of matured BOVINE OOCYTEs may not compromise their potency for further in vitro development.

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APA: Copy

KHOUB BAKHT, Z., HAJIAN, MAHDI, HOSSEINI, SEYED MORTEZA, FOROUZANFAR, MOHSEN, MOULAVI, FARIBA, ABEDI, PARVANEH, HOSSEINI, LALEH, KARAMI SHABANKAREH, H., & NASRESFAHANI, MOHAMMAD HOSSEIN. (2008). EFFECT OF DEMECOLICNE TREATMENT ON THE DEVELOPMENTAL COMPETENCY OF IN VITRO MATURED BOVINE OOCYTES. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 6(24), 447-457. SID. https://sid.ir/paper/81400/en

Vancouver: Copy

KHOUB BAKHT Z., HAJIAN MAHDI, HOSSEINI SEYED MORTEZA, FOROUZANFAR MOHSEN, MOULAVI FARIBA, ABEDI PARVANEH, HOSSEINI LALEH, KARAMI SHABANKAREH H., NASRESFAHANI MOHAMMAD HOSSEIN. EFFECT OF DEMECOLICNE TREATMENT ON THE DEVELOPMENTAL COMPETENCY OF IN VITRO MATURED BOVINE OOCYTES. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2008;6(24):447-457. Available from: https://sid.ir/paper/81400/en

IEEE: Copy

Z. KHOUB BAKHT, MAHDI HAJIAN, SEYED MORTEZA HOSSEINI, MOHSEN FOROUZANFAR, FARIBA MOULAVI, PARVANEH ABEDI, LALEH HOSSEINI, H. KARAMI SHABANKAREH, and MOHAMMAD HOSSEIN NASRESFAHANI, “EFFECT OF DEMECOLICNE TREATMENT ON THE DEVELOPMENTAL COMPETENCY OF IN VITRO MATURED BOVINE OOCYTES,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 6, no. 24, pp. 447–457, 2008, [Online]. Available: https://sid.ir/paper/81400/en

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