COMPARISON OF THE EFFECTS OF MYO-INOSITO ALONE OR IN COMBINATION WITH MEM VITAMINS ON EMBRYO DEVELOPMENT IN SANJABI SHEEP

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KAFILZADEH F.; KARAMI SHABANKAREH H.; SOLTANI L.; KAZEMINE JASEMI V.

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Journal: Anatomical Sciences Journal Year:2011 | Volume:8 | Issue:32-33 Page(s): 190-196

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Title

COMPARISON OF THE EFFECTS OF MYO-INOSITO ALONE OR IN COMBINATION WITH MEM VITAMINS ON EMBRYO DEVELOPMENT IN SANJABI SHEEP

Author(s)

KAFILZADEH F. | KARAMI SHABANKAREH H. | SOLTANI L. | KAZEMINE JASEMI V. | Issue Writer Certificate

Keywords

MYO–INOSITOLQ3

IN VITRO FERTILIZATIONQ3

EMBRYO DEVELOPMENTQ3

SANJABI SHEEPQ3

Abstract

Purpose: The objective of this study was to evaluate the effects of myo-inositol alone or in combination with MEM vitamins on EMBRYO DEVELOPMENT in SANJABI SHEEP.Materials and Methods: Sheep Cumulus Oocytes Complexes (COCs) were matured in vitro at 39ºC, in humidified 5% CO2 atmosphere for 22-24 h. There were three treatments, culture in synthetic oviductal fluid medium (treatment I), culture in synthetic oviductal fluid medium supplemented with1×MEM vitamins (treatment II), culture in synthetic oviductal fluid medium supplemented with myo-inositol (treatment III), COCs were then fertilized and cultured in vitro for 8 days when the ratios of in vitro EMBRYO DEVELOPMENT of the hatched blastocysts were assessed and compared with the control group.Results: The presence of myo-inpsitol significantly improved overall morula rates (34.39%) than that of control group (24.18%), but there was no difference between myo-inositol and 1×MEM vitamins in the percentage of embryos successfully developing to the morula stage (p<0.05). The addition of myo-inositol improved the mean blastocyst formation compared to the oocytes matured in medium containing no MEM (control) or 1×MEM vitamins (32.63, 13.96, and 21.06% respectively). However the mean percentage of cleavage rate was not substantialy different between treatments. conclusion: These results suggest that adding myo-inositol to SOF medium be more beneficial for subsequent sheep embryonic development.

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APA: Copy

KAFILZADEH, F., KARAMI SHABANKAREH, H., SOLTANI, L., & KAZEMINE JASEMI, V.. (2011). COMPARISON OF THE EFFECTS OF MYO-INOSITO ALONE OR IN COMBINATION WITH MEM VITAMINS ON EMBRYO DEVELOPMENT IN SANJABI SHEEP. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 8(32-33), 190-196. SID. https://sid.ir/paper/81429/en

Vancouver: Copy

KAFILZADEH F., KARAMI SHABANKAREH H., SOLTANI L., KAZEMINE JASEMI V.. COMPARISON OF THE EFFECTS OF MYO-INOSITO ALONE OR IN COMBINATION WITH MEM VITAMINS ON EMBRYO DEVELOPMENT IN SANJABI SHEEP. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2011;8(32-33):190-196. Available from: https://sid.ir/paper/81429/en

IEEE: Copy

F. KAFILZADEH, H. KARAMI SHABANKAREH, L. SOLTANI, and V. KAZEMINE JASEMI, “COMPARISON OF THE EFFECTS OF MYO-INOSITO ALONE OR IN COMBINATION WITH MEM VITAMINS ON EMBRYO DEVELOPMENT IN SANJABI SHEEP,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 8, no. 32-33, pp. 190–196, 2011, [Online]. Available: https://sid.ir/paper/81429/en

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