RAPID LATEX AGGLUTINATION INHIBITION REACTION TEST FOR MORPHINE IN URINE

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AKBARZADEH A.; NOUROUZIAN D.; FARAHMAND B.

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Journal Paper

Paper Information

Journal: Journal of Sabzevar University of Medical Sciences Year:2002 | Volume:9 | Issue:1 (23) Page(s): 10-19

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Title

RAPID LATEX AGGLUTINATION INHIBITION REACTION TEST FOR MORPHINE IN URINE

Author(s)

AKBARZADEH A. | NOUROUZIAN D. | FARAHMAND B. | Issue Writer Certificate

Keywords

MORPHINEQ2

IMMUNOASSAYQ3

LATEX AGGLUTINATIONQ4

Abstract

A simple and rapid IMMUNOASSAY of MORPHINE based on LATEX AGGLUTINATION inhibition reaction has been developed. MORPHINE-6-succinat is a MORPHINE derivative, and its BSA conjugates were synthesized and characterized. The hapten density in the conjugate was determined spectroscopically to be 8.2 mol/mol of BSA. It was found that MORPHINE-6- succinyl-BSA exhibits favorable features in terms of immunogenicity and immunochemical specificity. A LATEX AGGLUTINATION inhibition-reaction test (LAIRT) using DEAE-Cellulose purified rabbit IgG against MORPHINE-6- succinyl-BSA was found to give a sensitivity of 300 mg/ml of MORPHINE. Various commonly used drugs and narcotics at concentrations 0.1 mg/ml or less did not interfere with the test. Interference by normal urine was observed but it could be eliminated by the inclusion of 0.78% normal rabbit serum. The sensitized latex was stable at 4°C for at least 6 months .It was also stable to lyophilization and to at least four cycles of freezing and thawing. The total test time was 5 minutes. A comparison was made between the LAIRT and EMIT on 100 urine samples collected from people who referred to governmental narcotic clinic (Reference Lab. Tehran, Iran). While the EMIT showed 84 positives and 16negatives, the LAIRT gave 68 positives and 32 negatives. The two tests showed no significant difference (P<0.05).

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APA: Copy

AKBARZADEH, A., NOUROUZIAN, D., & FARAHMAND, B.. (2002). RAPID LATEX AGGLUTINATION INHIBITION REACTION TEST FOR MORPHINE IN URINE. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 9(1 (23)), 10-19. SID. https://sid.ir/paper/82640/en

Vancouver: Copy

AKBARZADEH A., NOUROUZIAN D., FARAHMAND B.. RAPID LATEX AGGLUTINATION INHIBITION REACTION TEST FOR MORPHINE IN URINE. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2002;9(1 (23)):10-19. Available from: https://sid.ir/paper/82640/en

IEEE: Copy

A. AKBARZADEH, D. NOUROUZIAN, and B. FARAHMAND, “RAPID LATEX AGGLUTINATION INHIBITION REACTION TEST FOR MORPHINE IN URINE,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 9, no. 1 (23), pp. 10–19, 2002, [Online]. Available: https://sid.ir/paper/82640/en

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