THE EFFECT OF HEPATOCYTE GROWTH FACTOR ON MOUSE OOCYTE IN VITRO MATURATION AND SUBSEQUENT FERTILIZATION AND EMBRYO DEVELOPMENT

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BAHADORI MOHAMMAD H.; AZARNIA MAHNAZ; GHASEMIAN FATEMEH

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Journal: Zahedan Journal of Research in Medical Sciences Year:2011 | Volume:13 | Issue:2 Page(s): 26-30

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Title

THE EFFECT OF HEPATOCYTE GROWTH FACTOR ON MOUSE OOCYTE IN VITRO MATURATION AND SUBSEQUENT FERTILIZATION AND EMBRYO DEVELOPMENT

Author(s)

BAHADORI MOHAMMAD H. | AZARNIA MAHNAZ | GHASEMIAN FATEMEH | Issue Writer Certificate

Keywords

HEPATOCYTE GROWTH FACTORQ2

IVMQ2

IVFQ2

EMBRYONIC DEVELOPMENTQ2

Abstract

Background: Oocyte invitro maturation is an enormously promising technology for the treatment of infertility, yet its clinical application remains limited owing to poor success rates. Therefore, this study was devised to evaluate the effect of HEPATOCYTE GROWTH FACTOR (HGF) on in vitro maturation of immature mouse oocytes and resulting embryos development.Materials and Method: Cumulus – oocyte complex and germinal vesicle were obtained from eighteen 6-8 weeks-old female NMRI mice 46-48 hours after administration of an injection of 5 IU PMSG (Pregnant Mares’ Serum Gonadotrophin). Oocytes were culture in TCM199 (Tissue culture medium-199) supplemented with dosages of 0, 10, 20, 50 and 100 ng/ml of HGF. After 24 hours, metaphaseІІ oocytes were co-incubated with sperms for 4-6 hours in T6 medium. Following isolation of two pronucleus embryos, cleavage of embryos was assessed in the same medium till blastocyst stage. The number of oocytes and embryos was recorded under an invert microscope and the rate of oocyte maturation, fertilization and embryos cleavage until blastocyst stage compared using of studentχ2 test.Results: In all compared groups, oocytes growth and embryos development rate in the 20 ng/ml of HGF treatment group was significantly higher (p<0.05) than the control group (p<0.05).Conclusion: 20 ng/ml of HGF improved the nuclear maturation and embryo development up to blastocyst stage during culture condition.

Cites

EFFECT OF ENDURANCE SWIMMING TRAINING DURING PREGNANCY ON HISTOLOGY AND APOPTOTIC INDEX OF RATS' LIVER

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APA: Copy

BAHADORI, MOHAMMAD H., AZARNIA, MAHNAZ, & GHASEMIAN, FATEMEH. (2011). THE EFFECT OF HEPATOCYTE GROWTH FACTOR ON MOUSE OOCYTE IN VITRO MATURATION AND SUBSEQUENT FERTILIZATION AND EMBRYO DEVELOPMENT. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 13(2), 26-30. SID. https://sid.ir/paper/84236/en

Vancouver: Copy

BAHADORI MOHAMMAD H., AZARNIA MAHNAZ, GHASEMIAN FATEMEH. THE EFFECT OF HEPATOCYTE GROWTH FACTOR ON MOUSE OOCYTE IN VITRO MATURATION AND SUBSEQUENT FERTILIZATION AND EMBRYO DEVELOPMENT. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2011;13(2):26-30. Available from: https://sid.ir/paper/84236/en

IEEE: Copy

MOHAMMAD H. BAHADORI, MAHNAZ AZARNIA, and FATEMEH GHASEMIAN, “THE EFFECT OF HEPATOCYTE GROWTH FACTOR ON MOUSE OOCYTE IN VITRO MATURATION AND SUBSEQUENT FERTILIZATION AND EMBRYO DEVELOPMENT,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 13, no. 2, pp. 26–30, 2011, [Online]. Available: https://sid.ir/paper/84236/en

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