EFFECTS OF GLP-1 ON Γ-AMINOBUTYRIC ACID AND SEROTONIN METABOLISM IN SYNAPTOSOMAL FRACTIONS FROM RAT HYPOTHALAMUS AND BRAIN STEM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NIKSERESHT M.; SANAE F.; OUJI A.A.; DELAVIZ H.A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Zahedan Journal of Research in Medical Sciences Year:2004 | Volume:6 | Issue:3 Page(s): 165-172

Download Full-Text

Persian Verion

View:

1,852

Download:

Cites:

Information Journal Paper

Title

EFFECTS OF GLP-1 ON Γ-AMINOBUTYRIC ACID AND SEROTONIN METABOLISM IN SYNAPTOSOMAL FRACTIONS FROM RAT HYPOTHALAMUS AND BRAIN STEM

Author(s)

NIKSERESHT M. | SANAE F. | OUJI A.A. | DELAVIZ H.A. | Issue Writer Certificate

Keywords

CLP-1. 5-HTQ4

GABA. HYPOTHALAMUSQ4

RATQ2

Abstract

Background: Glucagons - like peptide-I (7-36) amide (CLP-I) is the main product of the glucagon gene expression in intestinal L cells. CLP-I is released from the intestine into the circulation in response to the ingestion of food and is the most potent stimulator of glucose-induced insulin secretion. CLP-I receptors have also been detected in discrete areas of RAT brain and intracerebro ventricular injection of CLP-I has been shown to inhibit feeding in fasted RATs. Methods and Materials: This study is an in vitro study. Used RATs are male and their weight is 200 to 220 grams and each group has 7 RATs. In this study HPLC techniques were employed to evaluate the effects of CLP-I on monoamine metabolism in RAT brain as a neuropeptide. Synaptosoms were prepared from homogenates of combined hypothalamus and brain stem from RATs in each group. The synaptosomal pellets incubated for 15 minutes in ashaker bath at 37 C. The effect of CLP-I on synaptosoms was tested by adding 50 µL of CLP-1 5×10 -7 M to the incubation medium. For this group a control group was prepared by adding normal saline instead of GLP-I. The data were analyzed by T- test. Results: CLP-I decreased levels of serotonin (5-HT) by 20% (P<0.05) after 15 minutes of incubation with combined hypo thalamus and brain stem synaptosomes. Level of 5 hydroxyindolacetic acid (5-H1AA), the principal metabolite of 5-HT, and tryptophan the amino acid precursor of 5-HT decreased by 21% and 37 %( P<0.05) respectively. γ-Aminobutyric acid (CABA) and its amino acid precursor glutamic acid (Glu) were both quantities at the same conditions as above, but a operculum derivatization HPLC technique was used. The increase levels of GABA (14%) and Glu (6%) by GLP-I was not significant. Conclusions: The results suggest that decreased synaptosomal levels of 5-HT and 5-H1AA caused by GLP-I are due to diminished availability of tryptophan by the neuropeptide.

Cites

No record.

References

No record.

Cite

APA: Copy

NIKSERESHT, M., SANAE, F., OUJI, A.A., & DELAVIZ, H.A.. (2004). EFFECTS OF GLP-1 ON Γ-AMINOBUTYRIC ACID AND SEROTONIN METABOLISM IN SYNAPTOSOMAL FRACTIONS FROM RAT HYPOTHALAMUS AND BRAIN STEM. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 6(3), 165-172. SID. https://sid.ir/paper/84292/en

Vancouver: Copy

NIKSERESHT M., SANAE F., OUJI A.A., DELAVIZ H.A.. EFFECTS OF GLP-1 ON Γ-AMINOBUTYRIC ACID AND SEROTONIN METABOLISM IN SYNAPTOSOMAL FRACTIONS FROM RAT HYPOTHALAMUS AND BRAIN STEM. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2004;6(3):165-172. Available from: https://sid.ir/paper/84292/en

IEEE: Copy

M. NIKSERESHT, F. SANAE, A.A. OUJI, and H.A. DELAVIZ, “EFFECTS OF GLP-1 ON Γ-AMINOBUTYRIC ACID AND SEROTONIN METABOLISM IN SYNAPTOSOMAL FRACTIONS FROM RAT HYPOTHALAMUS AND BRAIN STEM,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 6, no. 3, pp. 165–172, 2004, [Online]. Available: https://sid.ir/paper/84292/en

Related Journal Papers