PRODUCING NO. IN MICE MACROPHAGES AND HUMAN MONOCYTES FOLLOWING CONFRONTATION WITH C.ALBICANS

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PAKZAD I.; ZAVARAN HUSSEINI A.; KHOSRAVI A.

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Paper Information

Journal: JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES Year:2002 | Volume:9 | Issue:32-33 Page(s): 18-24

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Information Journal Paper

Title

PRODUCING NO. IN MICE MACROPHAGES AND HUMAN MONOCYTES FOLLOWING CONFRONTATION WITH C.ALBICANS

Author(s)

PAKZAD I. | ZAVARAN HUSSEINI A. | KHOSRAVI A. | Issue Writer Certificate

Keywords

C.ALBICANSQ2

NITRIC OXIDEQ2

BALB/CQ4

MACROPHAGEQ3

Abstract

In this study BALB/C mice MACROPHAGEs and human monocytes were collected and cultured in six groups in 96-well microplates containing RPMI- 1640 separately. The first group involved MACROPHAGEs, C.ALBICANS and rIFN-y, whereas its control group (the second group) contained MACROPHAGEs, C.ALBICANS and NG-MMLA. The third group received MACROPHAGEs and C.ALBICANS whereas its control group (the fourth) included MACROPHAGEs, C.ALBICANS and NG-MMLA. The fifth group had only MACROPHAGEs and its control group (the sixth) involved MACROPHAGEs and NG-MMLA.The same protocol was repeated for human monocytes separated on ficoll and incubated in 96-plate for adherency. The plates were incubated in 5% Co2 for 4hs at 37.c, then supernatant of each well was removed and NO. production was assessed by Griess method.Macrophages and monocyetes were laysed by distilled water and 1% BSA and each well was plated on sabauraud dextrose agar. The number of colony forming unit (CFU)was registered after 48 hours of incubation at 37.c.Statistical analysis resulted in a difference between experimental and control groups in NO production and killing of C.ALBICANS (p<0.05).NO. production and candida killing percent was higher in experimental than that of the control groups (p<0.05). The results proved the role of NO. in candidacidal and candidastatic in mouse MACROPHAGEs and human monocytes.

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APA: Copy

PAKZAD, I., ZAVARAN HUSSEINI, A., & KHOSRAVI, A.. (2002). PRODUCING NO. IN MICE MACROPHAGES AND HUMAN MONOCYTES FOLLOWING CONFRONTATION WITH C.ALBICANS. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, 9(32-33), 18-24. SID. https://sid.ir/paper/90057/en

Vancouver: Copy

PAKZAD I., ZAVARAN HUSSEINI A., KHOSRAVI A.. PRODUCING NO. IN MICE MACROPHAGES AND HUMAN MONOCYTES FOLLOWING CONFRONTATION WITH C.ALBICANS. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2002;9(32-33):18-24. Available from: https://sid.ir/paper/90057/en

IEEE: Copy

I. PAKZAD, A. ZAVARAN HUSSEINI, and A. KHOSRAVI, “PRODUCING NO. IN MICE MACROPHAGES AND HUMAN MONOCYTES FOLLOWING CONFRONTATION WITH C.ALBICANS,” JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, vol. 9, no. 32-33, pp. 18–24, 2002, [Online]. Available: https://sid.ir/paper/90057/en

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