EVALUATION FREQUENCY OF TEM, VEB AND PER GENS EXTENDED SPECTRUM BETA LACTAMASE PRODUCING OF ESCHERICHIA COLI STRAINS ISOLATED FROM URINARY TRACT INFECTIONS IN ILAM CITY

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VALADBEIGI T.; CHALABZARDI M.

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Journal: JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES Year:2016 | Volume:24 | Issue:1 Page(s): 55-63

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Title

EVALUATION FREQUENCY OF TEM, VEB AND PER GENS EXTENDED SPECTRUM BETA LACTAMASE PRODUCING OF ESCHERICHIA COLI STRAINS ISOLATED FROM URINARY TRACT INFECTIONS IN ILAM CITY

Author(s)

VALADBEIGI T. | CHALABZARDI M. | Issue Writer Certificate

Keywords

BETA-LACTAMASEQ1

TEMQ1

VEBQ2

PERQ1

Abstract

Introduction: Escherichia coli are the mostcommon agent of urinary tract infection.One of mechanisms of resistance to the beta-lactam antibiotics beta lactamase is enzyme production. Including beta lactamase٫ as BETA-LACTAMASE producing of genes of the TEM, PER and VEB in these bacteria can be named. Purpose of presentstudy evaluation frequency of foresaid gensextended spectrum beta lactamaseproducing of Escherichia coli strainsisolated from urinary tract infections was incity Ilam.Materials & methods: A total of 100 strainsof E.coli were isolated from sample surinary tract and were identified using biochemical tests. Then antibioticsusceptibility was determined by diskdiffusion method. Finally testing combineddisk detects ESBL producing strains and MIC of strains to antibiotic CAZ and CTXby Micro broth dilution method. PCR with specific primers was used for determiningthe presence of blaPER, blaVEB and blaTEM genes.Findings: Combined disk test showed 40 strains (40%) to be ESBL producing. Of the 40 ESBL producing strains, MIC force ftazidime in the dilutions 16, 18 samples, 32, 9 samples 64, 16 samples, 128, 1sample, 256, 2 samples were reported. MIC for cefotaxime in the dilutions16,13 samples, 32, 9samples, 64, 5 samples, 128, 8 samples, 256, 4 samples weredetermined. Of the 40 strains ESBL the frequency of TEM was 52.5%; however, blaPER and blaVEB genes were notdetected among ESBL producing isolates.Discussion & Conclusions: Frequency of Escherichia coli producing ESBL was 40%in city of Ilam and TEM gene in ESBLproducing E.coli isolated is the mostcommon gene in Ilam City.

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APA: Copy

VALADBEIGI, T., & CHALABZARDI, M.. (2016). EVALUATION FREQUENCY OF TEM, VEB AND PER GENS EXTENDED SPECTRUM BETA LACTAMASE PRODUCING OF ESCHERICHIA COLI STRAINS ISOLATED FROM URINARY TRACT INFECTIONS IN ILAM CITY. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, 24(1), 55-63. SID. https://sid.ir/paper/90138/en

Vancouver: Copy

VALADBEIGI T., CHALABZARDI M.. EVALUATION FREQUENCY OF TEM, VEB AND PER GENS EXTENDED SPECTRUM BETA LACTAMASE PRODUCING OF ESCHERICHIA COLI STRAINS ISOLATED FROM URINARY TRACT INFECTIONS IN ILAM CITY. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;24(1):55-63. Available from: https://sid.ir/paper/90138/en

IEEE: Copy

T. VALADBEIGI, and M. CHALABZARDI, “EVALUATION FREQUENCY OF TEM, VEB AND PER GENS EXTENDED SPECTRUM BETA LACTAMASE PRODUCING OF ESCHERICHIA COLI STRAINS ISOLATED FROM URINARY TRACT INFECTIONS IN ILAM CITY,” JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, vol. 24, no. 1, pp. 55–63, 2016, [Online]. Available: https://sid.ir/paper/90138/en

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