PCR DETECTION OF PER & VEB & SHV AND TEM BETA-LACTAMASES IN MULTIDRUG RESISTANT P. AERUGINOASA ISOLATED FROM WOUND INFECTIONS IN TWO HOSPITALS OF TEHRAN

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SHAH CHERAGHI FERESHTEH; NIKBIN V.A.S.; SHOURAJ F.

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Journal: Iranian Journal of Medical Microbiology Year:2008 | Volume:1 | Issue:4 Page(s): 21-27

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Title

PCR DETECTION OF PER & VEB & SHV AND TEM BETA-LACTAMASES IN MULTIDRUG RESISTANT P. AERUGINOASA ISOLATED FROM WOUND INFECTIONS IN TWO HOSPITALS OF TEHRAN

Author(s)

SHAH CHERAGHI FERESHTEH | NIKBIN V.A.S. | SHOURAJ F. | Issue Writer Certificate

Keywords

SHVQ4

TEMQ3

PERQ3

VEBQ4

BETA-LACTAMASEQ4

P. AERUGINOSAQ4

Abstract

Background and objectives: Class A serine BETA-LACTAMASEs such as SHV, TEM, PER and VEB are among the most important resistance determinants emerging in gram negative bacterial pathogens. PER BETA-LACTAMASE is an enzyme of notable clinical importance due its ESBL (extended spectrum BETA-LACTAMASEs) activity and fully characterized in P. AERUGINOSA. The present investigation was under taken to assess the prevalence of BETA-LACTAMASE genes belonging to SHV, TEM, PER and VEB in P. AERUGINOSA isolates from wound infections in two hospitals of Tehran.Material and Methods: Totally 100 isolates of Multi Drug Resistant P. aeroginosa from wound infections were collected over one year PERiod in two hospitals of Tehran. Susceptibility to antibiotics and MICs for ceftazidime and imipenem of these 100 isolates were assessed using disc diffusion and microdilution methods, respectively. Isolates showing MIC³16 microgram/ml for ceftazidime were subjected to PCR using four primer sets including SHV, TEM, PER and VEB types of BETA-LACTAMASEs.Results: Only 6 of 100 studied isolates showed MIC³4 for imipnem. Resistance to ceftazidime was determined in 42% of the isolates and 46% of the isolates showed MIC³16 microgram/ml for ceftazidime. PCR assay indicated that out of 46 isolates showing MIC³16 microgram/ml for ceftazidime, 28%, 11%, 13% and 11% were positive for SHV, TEM, PER and VEB BETA-LACTAMASE genes.Conclusion: Imipenem was the most effective antibiotic against multidrug resistant P. aeroginosa isolates from wound infections in this research. SHV BETA-LACTAMASE was more observed than three other studied genes among the isolates recovered from wound in two studied hospitals of Tehran. It is the first report of bla PER and bla VEB in P. AERUGINOSA in Iran.

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APA: Copy

SHAH CHERAGHI, FERESHTEH, NIKBIN, V.A.S., & SHOURAJ, F.. (2008). PCR DETECTION OF PER & VEB & SHV AND TEM BETA-LACTAMASES IN MULTIDRUG RESISTANT P. AERUGINOASA ISOLATED FROM WOUND INFECTIONS IN TWO HOSPITALS OF TEHRAN. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 1(4), 21-27. SID. https://sid.ir/paper/132532/en

Vancouver: Copy

SHAH CHERAGHI FERESHTEH, NIKBIN V.A.S., SHOURAJ F.. PCR DETECTION OF PER & VEB & SHV AND TEM BETA-LACTAMASES IN MULTIDRUG RESISTANT P. AERUGINOASA ISOLATED FROM WOUND INFECTIONS IN TWO HOSPITALS OF TEHRAN. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2008;1(4):21-27. Available from: https://sid.ir/paper/132532/en

IEEE: Copy

FERESHTEH SHAH CHERAGHI, V.A.S. NIKBIN, and F. SHOURAJ, “PCR DETECTION OF PER & VEB & SHV AND TEM BETA-LACTAMASES IN MULTIDRUG RESISTANT P. AERUGINOASA ISOLATED FROM WOUND INFECTIONS IN TWO HOSPITALS OF TEHRAN,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 1, no. 4, pp. 21–27, 2008, [Online]. Available: https://sid.ir/paper/132532/en

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