EFFECT OF COBALT SULFATE INTRAPERITONEAL INJECTION ON THE QUANTITATIVE VALUES OF THE KINDS OF WHITE BLOOD CELLS AND INITIATION OF LIVER FIBROSIS IN RAT

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MOZAFARIAN F.; ZAKERIFAR A.; JALALLOU NAHID; PASBAKHSH P.; MEHBOD S.A.A.

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Journal: Annals of Military and Health Sciences Research Year:2007 | Volume:5 | Issue:3 (SERIAL NUMBER 19) Page(s): 1317-1323

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Title

EFFECT OF COBALT SULFATE INTRAPERITONEAL INJECTION ON THE QUANTITATIVE VALUES OF THE KINDS OF WHITE BLOOD CELLS AND INITIATION OF LIVER FIBROSIS IN RAT

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Keywords

INTRAPEIRTONEAL INJECTIONQ3

COBALTQ3

LIVERQ3

RATQ2

WHITE BLOOD CELLQ3

Abstract

Background: COBALT 60 is used in sterilization and there are many other variable uses of COBALT sulfate in the synthesis of alloys in air-space army dependant manufactures and also regard of its domestic uses and importance of COBALT soils after bombardment we decided to investigate this metal. And because of WBC involvement in much important system 'disorders we decided to investigate effect of COBALT sulfate on WBC amount.Materials & Methods: In this experimental study: 47 four weeks old wistar RATs with body weight 224 (male) and 1493 (female) were purchased from Pasteur Institute. One month was considered for their adaptation with animal laboratory with 20-26 centtigrade and 12hour dark and 12 hours light cycle. The animals were divided in every sex into 5 groups.4 Doses 1.0, 3.5, 8.0, 11.5miligramper kilogram body weight were prepared and were administered intraperitoneally for 1, 2, 3 and 4 groups. The fifth group receive distilled water with the same injection rhytme (2 milliliters, three times in the week). After 9 weeks they were anesthetized and blood from heart and bone marrow from the femur were sampled. The LIVERs of the RATs were dissected free and prepared for light microscopy. Their blood parameters were measured and the smears of bone marrow and blood were stained with Wright and Giemsa stain. LIVERs were stained by Hematoxicilin & Eosin and Van Giesson stain.The slides were studied and the results were analysed with Minitab software and Mann Withney test. Results: Eosinophil, basophil did not have any significant change in the groups. LIVER pathologic figures like fibrosis and leukocyte infiltration has been seen in the fourth male group (P=0.02).Conclusion: The precise analytical changes of WBC in this exposure needs to study more in high quantity The results presents the possibility of WBC increase.

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APA: Copy

MOZAFARIAN, F., ZAKERIFAR, A., JALALLOU, NAHID, PASBAKHSH, P., & MEHBOD, S.A.A.. (2007). EFFECT OF COBALT SULFATE INTRAPERITONEAL INJECTION ON THE QUANTITATIVE VALUES OF THE KINDS OF WHITE BLOOD CELLS AND INITIATION OF LIVER FIBROSIS IN RAT. ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH, 5(3 (SERIAL NUMBER 19)), 1317-1323. SID. https://sid.ir/paper/96564/en

Vancouver: Copy

MOZAFARIAN F., ZAKERIFAR A., JALALLOU NAHID, PASBAKHSH P., MEHBOD S.A.A.. EFFECT OF COBALT SULFATE INTRAPERITONEAL INJECTION ON THE QUANTITATIVE VALUES OF THE KINDS OF WHITE BLOOD CELLS AND INITIATION OF LIVER FIBROSIS IN RAT. ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH[Internet]. 2007;5(3 (SERIAL NUMBER 19)):1317-1323. Available from: https://sid.ir/paper/96564/en

IEEE: Copy

F. MOZAFARIAN, A. ZAKERIFAR, NAHID JALALLOU, P. PASBAKHSH, and S.A.A. MEHBOD, “EFFECT OF COBALT SULFATE INTRAPERITONEAL INJECTION ON THE QUANTITATIVE VALUES OF THE KINDS OF WHITE BLOOD CELLS AND INITIATION OF LIVER FIBROSIS IN RAT,” ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH, vol. 5, no. 3 (SERIAL NUMBER 19), pp. 1317–1323, 2007, [Online]. Available: https://sid.ir/paper/96564/en

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