مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

Identification of molecular pathways and protein-protein interactions in adipose tissue-derived mesenchymal stromal cells (ASCs) under physiological oxygen concentration in a diabetic rat model

Pages

  155-163

Abstract

 therapy. However, it is well known that diabetes mellitus (DM) alters ASCs’ functionality. The majority of in vitro studies related to ASCs are developed under non-physiological oxygen conditions. Therefore, they may not reflect the full effects of DM on ASCs, in vivo. The main aim of the current study is to identify molecular pathways and underlying biological mechanisms affected by diabetes on ASCs in physiological oxygen conditions. Materials and Methods: ASCs derived from healthy (ASCs-C) and diabetic (ASCs-D) rats were expanded under standard culture conditions (21% O2) or cultured in physiological oxygen conditions (3% O2) and characterized. Differential gene expressions (DEGs) of ASCs-D with respect to ASCs-C were identified and analyzed with bioinformatic tools. Protein-protein interaction (PPI) networks, from up-and down-regulated DEGs, were also constructed. Results: The bioinformatic analysis revealed 1354 up-regulated and 859 down-regulated DEGs in ASCs-D, with 21 and 78 terms over and under-represented, respectively. Terms linked with glycosylation and ribosomes were over-represented and terms related to the activity of RNA-polymerase II and transcription regulation were under-represented. PPI network disclosed RPL11-RPS5 and KDR-VEGFA as the main interactions from up-and down-regulated DEGs, respectively. Conclusion: These results provide valuable information about gene pathways and underlying molecular mechanisms by which diabetes disturbs ASCs biology in physiological oxygen conditions. Furthermore, they reveal, molecular targets to improve the use of ASCs in autologous transplantation.

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    APA: Copy

    Paco Meza, Luis Miguel, Carmona, MDolores, Canadillas, Sagrario, Lopez Diaz, Ana, Munoz Lopez, Francisco, Jimenez Arranz, Alvaro, Guler, Ipek, & Herrera, Concha. (2022). Identification of molecular pathways and protein-protein interactions in adipose tissue-derived mesenchymal stromal cells (ASCs) under physiological oxygen concentration in a diabetic rat model. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 25(2), 155-163. SID. https://sid.ir/paper/975771/en

    Vancouver: Copy

    Paco Meza Luis Miguel, Carmona MDolores, Canadillas Sagrario, Lopez Diaz Ana, Munoz Lopez Francisco, Jimenez Arranz Alvaro, Guler Ipek, Herrera Concha. Identification of molecular pathways and protein-protein interactions in adipose tissue-derived mesenchymal stromal cells (ASCs) under physiological oxygen concentration in a diabetic rat model. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2022;25(2):155-163. Available from: https://sid.ir/paper/975771/en

    IEEE: Copy

    Luis Miguel Paco Meza, MDolores Carmona, Sagrario Canadillas, Ana Lopez Diaz, Francisco Munoz Lopez, Alvaro Jimenez Arranz, Ipek Guler, and Concha Herrera, “Identification of molecular pathways and protein-protein interactions in adipose tissue-derived mesenchymal stromal cells (ASCs) under physiological oxygen concentration in a diabetic rat model,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 25, no. 2, pp. 155–163, 2022, [Online]. Available: https://sid.ir/paper/975771/en

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