PRODUCTION OF COLLAGEN TYPE I AND ITS USING IN MEDICAL PRACTICE

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RAHIMI MOHAMMAD KARIM; KHAJAVI RAMIN; MAHDAVI HEZAVEH MOHAMMAD AMIN; EBRAHIMI HOUR ELNAZ

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Journal Paper

Paper Information

Journal: MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY Year:2011 | Volume:20 | Issue:4 Page(s): 246-251

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Title

PRODUCTION OF COLLAGEN TYPE I AND ITS USING IN MEDICAL PRACTICE

Author(s)

RAHIMI MOHAMMAD KARIM | KHAJAVI RAMIN | MAHDAVI HEZAVEH MOHAMMAD AMIN | EBRAHIMI HOUR ELNAZ | Issue Writer Certificate

Keywords

SKIN AND DEBRIS OF FISHQ3

EXTRACTION METHODQ2

COLLAGEN TYPE IQ3

MEDICAL PRACTICESQ3

Abstract

Background: Collagen is a protein with specific importance in pharmacy and medicine. The aim of this study was extraction of COLLAGEN TYPE I by optimum using of Iranian fishes of Persian Gulf and also debris of Shilat industry.Materials and methods: Extraction of COLLAGEN TYPE I from the proper specimens was performed by two methods of acidic and alkaline. In acidic method, first, the lipids separated, then, by using HCL 5%, calcium elements were extracted. The pH of medium neutralized and hydrolysis was performed in high stream water vapor. The material have been filtered and in the vicinity of heat in vacuum state collagen concentrated. Then the sheets of collagen, grinding was done. In the alkaline method, the specimens were treated by sodium hydroxid 4% for 3 weeks at room temperature.Results: The rate of separated collagen from fish hasn’t significant difference between alkaline and acidic methods. For example, mean rate of 20.81% for collagen extraction from skin of fishes in alkaline method and mean of 19.76% in acidic method was detected. The collagen specimens extracted by these methods have equal or higher quality in comparison with specimens extracted from other sources.Conclusion: According to costless of primary materials, such as debris of fishes, in the production of collagen in Iran and import of collagen is expensive, domestic production is saving money.

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