The aerial parts of Thymuskotschyanus Boiss & Hohen were collected in three stages of plant growth (before, at the beginning and complete flowering) from research station of Sirachal. The essential oils of plant materials were isolated by steam-, hydro- and water-steam distillation from the aerial parts. The oils were analyzed by capillary GC and GC-MS. So, the effect of distillation methods and stages of plant growth on oil content and composition of Thymus kotschyanus was investigated. The oil yield, related to distillation method and stage of plant growth, was 0.28-1.80% w/w (the highest for complete flowering stage by hydrodistillation method). The main constitllents in all of the oils were carvacrol (46.74- 1.23%), thymol (7.51- 26.92 %), g-terpinene, p-cymene and borneol.