A significant amount of work has been done to better understand the barriers to knowledge flow and models of knowledge management cycle; this was intended to help in assessing the progress of KM initiatives in the firm. Using organizational knowledge better and more effectively in an organized and well-managed way, along with its combination in the organization’s culture would lead into a significant progress in organizations in terms of culture, social and economy. Although knowledge flow is invisible it works with any cooperative team, whether used it intentionally or not. It has been defined as a process of knowledge passing between people or knowledge processing mechanisms. The actors and tools of knowledge flow may become barriers if they are not handled properly. Temporal limitations, lack of staff motivation, and lack of incentives occur. In addition, lack of cross departmental cooperation, lack of absorptive capacity, hierarchical status, and physical distance are all obstacles to knowledge sharing. Therefore, recognizing the barriers of knowledge flow and efforts to remove them will lead into an easier knowledge flow trend. In this paper, through extracting recognized key barriers in resources, assimilation and localizing them in the country, we have tried to analyze their importance and occurrence probability based on knowledge management cycle including knowledge acquire, knowledge share, and knowledge application. Therefore one of the experts of municipality service organizations was used and experts’ judgment along with Multiple Attribute Decision Making techniques, especially TOPSIS techniques Algorithm, was used for the purpose of analysis. Finally, the most important barriers were found from the viewpoint of knowledge management and knowledge cycle steps.