To investigate the effect of different levels of salinity and growth promoting bacteria on yield and yield components of barley an experiment conducted in research field of Islamic Azad University Karaj branch at Mahdasht in 1389-1388 crop year as factorial in the basis of completely randomized blocks design in four replications. The first factor was salt concentrations using sodium chloride in four levels (0, 50, 100, 150 mM) and the second factor with four levels of bacteria strains including Azotobacter, Azosprillium, mixed of two bacteria, control (no inoculation). Seeds of Nosrat cultivar after inoculation with respected strains were planted in pots. During the plant growth period, soil moisture of pots were held at field capacity by adding water. Index of growth including plant height, peduncle length, spike length, spike weight, ear number, grain number per spike, 1000-grain weight, grain yield, biomass and harvesting indexe were determined. The results showed that, the effect of salinity treatments on peduncle length was significant at the 1% probability level, and the interaction of salinity and treatment of bacterial on ear weight, ear number, grain number per spike, grain yield, biomass and harvest index were significant at 1% level of probability and in plant height, spike length and grain weight were significant at 5% level. The traits related to yield and maximum yield conducted in s1b3 treatment (control, mixed two bacteria) with amount of (64.2 grams per unit area) and 20% yield increasing observed than control (s1b4) and lowest yield (53.1 gram per unit area) gained in control and Azotobacter consumption in the other hand 31% yield reduction compared to the control (s1b4). Salinity reduced grain yield and using of Azotobacter and Azosprillium can helps to increase barley grain yield in these conditions.