In order to study of the effect of inbreeding on economic traits, 22494 records were collected from 10 commercial silkworm lines of Iran Silkworm Research Center including Chinese lines of 32, 104, 152, 110, 154 and Japanese lines of 31, 103, 107, 151, 153 during 4 generations from 2005 to 2007. In the linear statistical model, inbreeding was considered as covariable along with the fixed effects of line, sex and generation. All the effects included in the model were highly significant on cocoon weight, cocoon shell weight and cocoon shell percentage (P>0.0001) except inbreeding covariable for cocoon shell weight. Lsmean method was used for mean comparisons. Mean differences among levels of fixed effects and covariables were significant in all the traits (P>0.0001) so that Chinese lines of 32, 152, 104 and Japanese lines of 153, 103, 31 showed good performance. The highest heritability for all the traits was observed in 31 line and the lowest in 153 line. For cocoon shell percentage, the lowest values belonged to 152, 104 and 32 lines. The average inbreeding coefficient was 0 in the first and second generations and 0.03644 and 0.07327 in third and fourth ones. This demonstrated that inbreeding coefficient becomes twice in the fourth generation due to not controlling relative matings.