The present research is mainly focused on how to standardize and prepare a short form for MMPI-2 questionnaire among Iranian university students. The sample consists of 3578 college students (1763 male and 1815 female) at different educational levels (AA, BA, MA, and PhD) from eight provinces, who were selected via multistage cluster sampling procedure. An Iranian and shortened version of MMPI-2 consisting of 370 questions was used. To investigate the construct validity of Iranian Shortened Version of MMPI-2, and to evaluate its reliability the principal components factor analysis and Oblimin oblique rotation was used. Results showed that Cronbach's Alpha coefficient as an index of reliability was 0.84 at first. This coefficient was increased to 0.96 through the omission of some questions with a weak co-efficient correlation. Among the remaining 156 questions, thirteen factors were extracted using factor analysis, Oblimin oblique rotation, skewing diagram and explained variance percentile. These factors, as a whole, explained 40.15 percent of the total variance of the variables. The naming of the factors was according to the original MMPI-2 including: Psychasthenia (PT), Frequency (F), Hypomania (Ma), Schizophrenia (Sc), Correction (K), Hypochondriasis (Hs), Hysteria (Hy), Paranoia (Pa), Depression (D), Lie (L), Psychopathic deviate (Pd), Social introversion-extroversion (Si), and Masculinity-Femininity (Mf). General findings indicate that the shorted form of MMPI-2 qualifies psychometric properties for clinical use and research activities in Iranian culture, especially in university settings.