Background and Objective: Escherichia coli pathogenic strains are considered as the main cause of intestinal and extra-intestinal diseases. This study aimed to identify and evaluate the dominant pathotypes of diarrheagenic E. coli isolates among children sufering from diarrhea in Khoram Abad city, Iran. Subjects and Methods: In this current study, 578 diarrhea samples of the children less than 10 years old were collected in Khoram Abbad city. After culture and isolation, the pathogenic groups of diarrheagenic E. coli (DEC) isolates including four pathotypes of Enterotoxigenic E. coli, Enteropathogenic E. coli, Enteroinvasive E. coli, and Shiga toxigenic E. coli were investigated by PCR. Disk diffusion method (Kirby Bauer) was used for detection of antimicrobial sensitivity of the isolates. Results: Out of 578 stool samples collected 81 isolates were DEC. The frequencies of the isolates were respectively: Enterotoxigenic E. coli (15. 1%), enteropathogenic E. coli (14%), Enteroinvasive E. coli (5%), and Shiga toxigenic E. coli (9. 7%). The highest antibiotic resistance levels were respectively reported to: amoxicilin (78%), tetracycline (74. 1%), co-trimoxazole (64. 2%), nalidixic acid (56. 8%), streptomycin (46. 9%), minocycline (38. 3%), and ciprofloxacin (16. 1%). Multiple drug resistance was found in 30. 9% of the isolates. Conclusion: Enterotoxigenic E. coli is one of the most common diarrheagenic agent affecting the children less than 10 years old in this region. Therefore, such diseases are preventable by controlling water and food hygiene especially in warm seasons.