The effects of Biomar, Calf heart, Tubifex, Gammarus and Artemia on some blood serum biochemical factors in Pangasius hypophthalmus were studied. This study was counducted during 8 weeks and 5 treatments were included: treatment 1: Biomar, treatment 2: Calf heart, treatment 3: Gammarus, treatment 4: Artemia, treatmeent 5: Tubifex. Amount of food required according to the weight of the biomass and 10 % of body weight four times a day in 8:00, 12:00, 16:00 and 20:00 hours were added to each aquarium. Physico-chemical parameters such as oxygen, hardness, pH, temperature were 5.5-6, 370 dh, 8 and 29±2oC, respectively. Finally, after about weight and length of test fish in each aquarium statistical calculations values of growth indicas were calculated according to the formula. Analyze data and draw graphs respectively by computer software SPSS 13 and Excel 2003 were performed. According to the results of this study there were significant differences in the value of glucose, triglyceride, cholesterol, calcium, albumin, total protein, phosphorus iron and alanin aminotransferase (P<0.05). However, there were no significant deifferences the value of aspartate aminotransferase and alkaline phosphatise (P>0.05). Based on the obtained results it can be declared that different diets could improve some blood serum biochemical factors in Pangasius hypophthalmus.