The aim of this study was to compare the effects of high-intensity interval training (HIT) and moderate-intensity continuous training (MCT) on maximal oxygen uptake (VO2max), ventilatory threshold (VT), respiratory compensation point (RCP), 3000-m running time (3000-m RT), peak (PPO) and mean power output (MPO). Therefore, twenty two collegiate soccer players participated in this study, voluntary. They were randomly divided into HIT group (12) and MCT group (12). Each training session involved 10 min warm-up, HIT program (6 to 10 ´ 30 s all-out sprints with 4 min recovery) or MCT (45 min running at 70% VO2max), and 5 min cool down performed 3 per week for 8 consecutive weeks. After training intervention, VO2max (7.6% vs. 8.9%), VT (4.5% vs. 3.9%), and RCP (4.2% vs. 3.8%) increased, and 3000-m RT decreased (-4.4% vs. -4.2%) significantly in HIT and MCT groups, respectively (p £ 0.05). There was no difference between the two groups for these variables (p > 0.05). Also, there was only an improvement for PPO (8.3%) and MPO (10.9%) in HIT group (p ≤ 0.001). It seems that HIT program with present training protocol, result in similar adaptations for cardiorespiratory fitness and endurance performance, compare with MCT program. Also, HIT program increases anaerobic capacity, simultaneously.