Beta-hydroxy-beta-methylbutyrate (HMB) has been extensively used as an ergonomic aid; particularly among bodybuilders and strength/power athletes. Studies have demonstrated that, in combination with resistance training, HMB supplementation resulted in increased muscular strength and lean mass and tended to decrease fat mass and indicators of muscle damage. While numerous studies have supported the efficacy of HMB, there have been conflicting results. Therefore, with the purpose of studying HMB supplementation effects on some of biochemical factors, 16 volunteer wrestlers selected and divided to two supplementation and placebo groups which along with their resistance training consume their prescribed capsules (3 gr per day). Before and after the training period, LDL, HDL, LDH, CPK, Ca, BUN, total cholesterol, and triglyceride evaluated and compared. Results showed that in both groups there are no significant changes in CPK, BUN, LDL, HDL, triglyceride and total cholesterol. There is a significant decrease in LDH (p=.005, p=.029, respectively) and increase in Ca concentration (p=.004, p=.049, respectively) in both groups although the changes in supplementation group was significantly greater.