The present research was done to investigate the Biochemical composition of (Protein, fat, Ash and Moisture), Fatty acid profiles and nutritional value of cultured Bighead carp of Mazandaran province. In this study caught 15 samples were sampled from 3 farms in Zaghemarz, Fereydoonkenar, Nashtarood and prepared by skinning and deboning. The results showed that Oleic acid (C18:1 n-9) and Arachidic acid (C20:0) were higher and lower amounts, respectively. Also, some indexes such as PUFA/SFA ratio, w-3, w-6, PI (polyen index), w-3/w-6 ratio, EPA+DHA and (OPI) Oxidation Progress Index as EPA+DHA/C16 were accounted. Contents of Total Protein, fat, moisture and fillet energy had highest amounts in Fereydoonkenar region and lowest in Zaghemarz region. The results also showed that there is no different between biochemical composition, statistically (P>0.05) but fillet energy showed difference (P<0.05). According to results cultured Bighead carp have more biological values due to high level protein and poly unsaturated fatty acids (PUFA) as a marine product.