This study aimed to comparative study of sargassum and canola meals as protein sources and determines the best replacement ratio in growing of white-leg shrimp (Litopenaeus vannamei). Since, juvenile shrimp with an average initial weight of 6.2±0.3g was performed from Jask hatchery and transferred to Off- Shore Research Center- Chabahar using standard method. After one week adaptation, eight experiment diets, all iso-nitrogenus (33% protein) and iso-caloric(3450 Kcal. 100 g-1 Dry weight of feed, each with 3 replicates including control (no canola and no sargassum meals), four treatment levels of 25, 50 and 75 and 100 percent of canola cake meal and 3 treatment levels of 5, 10 and 15% sargassum meal, were given 240 shrimp which they introduced to 24 plastic tank( 300 L.), 4 times per day, 8 am and 12, 16 and 29 pm, The average water temperature 29.60±0.60oC and salinity of 33±0.8 g.L-1. At the beginning of culture period, the feed were given based on 3% of BW and then ad libitum, growth parameters and survival rates were measured at 10 days interval. At the end of experiment, meantime measuring of biometrical parameters of all shrimps, 3 shrimps from each replicate were moved to laboratory to colorimetric, cholesterol determination and Caracas analysis. The results showed that even 100% canola and 15% sargassum meals can be replace as protein sources of shrimp diets without any adverse effect on growth and survival performances and there are not any differences between growth and survival rates of shrimp statistically (P<0.05). The diets containing sargassum meal specifically 15% changed shrimp color to pink and reduced the cholesterol shrimp content statistically (P<0.05), while the diets containing canola meals cannot The highest protein content were measured in Caracas shrimp of control group statistically while there are not any differences between treatments shrimp Caracas proteins statistically (P>0.05).