Ecophytochemistry journal of Medicinal Plants
Year:2014 | Volume:1 | Issue:4 (4)|Papers Count:9

Ferulago angulata, Bunium persicum and Dorema aucheri belongs to Apiaceae family were collected at summer 2012 from Bradsir in Kerman province during to fruiting stage. The essential oils of Bunium persicum and Ferulago angulata seeds were obtained by hydrodistillation using a Clevenger type apparatus and in Dorema aucheri was extracted by n-hexane and were analyzed by GC/MS. Diethylphetalate (79.15%) and dibuthylphethalate (5.73%) were the most components of Dorema aucheri, whereas b-ocimene (40.02%%), a-phelandrene (14.47%) and b-phelanderene (14.43%) in Ferulaga angulata oil and 3-caren-10-ol (27.99%), g-terpinene (23.93%) and cumin aldehyde (20.53%) were the most constituents in Bunium persicum oil, respectively.

Ferula gummosa Boiss. belongs to Apiaceae family is a perennial, endemic, monocarpic and useful medicinal-industrials plant in mountainous regions of Khorasan province. In this research and in many field observation different parts of Ferula gummosa (leaf, flower and root) were collected from two natural regions in Razavi Khorasan province (1760-2010 m), then ecological requirements and ethno pharmacology data were obtained and recorded by rural healer (67 years). Total phenol (TP) and total flavonoids (TF) of methanolic extracts were measured by spectrophotometery and antioxidant activity was measured by scavenging free radicals of DPPH. Results showed that the resin of the plant roots have been used by rural people as anti spasm, sedative and anti-infection to treat of migraine, dysmenorrheal and diarrhea. Total phenol and total flavanoid were reported 18.16 mg GAE g-1 and 15.20 mg QUE g-1, respectively and the highest content of antioxidant activity belonged to methanolic extract of flowers especially in Neishabour region (84.5%).

Plantago ovate Forsk. is an important medical plant belonging to plantaginaceae family which has been wild grows and used in many some parts of the Golestan province. This study was performed to compare the morphological charactristics and the mucilage content of 4 accessions of Plantago ovata in Golestan province (Gharemakhr, Douzolom, Incheh boron and Aramgah) based on randomized complete block design with four replications in spring of 2011. Morphological characteristics of accessions (Plant height, spike length, number of grains per spike and leaf length), thousand seed weight weighing and measuring inflation factor were recorded in their habitats and their mucilage extraction were performed at the laboratory of medicinal plants research center in Shahed University. Results showed that the leaf length and thousand seed weight were significantly different at the 1% level and the spike, spike length and inflation factors were significantly different at the 5% level. Differences between plant height, number of grains per spike, inflation per gram mucilage and mucilage content was not significant among the accessions. Generally, results showed that the highest amount of musilage was observed in Gharamakher ecotype (0.216 g) and so the survey of compare means shown that maximum of swell index was obtained in Gharamakher and Aramgah (14.25 ml). The Incheboron ecotype had minimum amount in swell index by 13.75 ml. Gharemakhras region had the top accession is introduced for all traits and it can be used in breeding programs.

Identification and plant speciation survey were the base ecological researches to keeping of general resources in per region. Rineh region (2300 m), with 700 hectares area from 79 km of Amol-Tehran road in South of Mazandaran province, on the north slops of the Alborz Mountain. In this study and in many field observation, 70 plant species of medicinal plants belongs to 46 genus and 21 family were collected and identified by Raunkiaea, Zohary and Takhtajan methods, in May to July 2013. Results showed that Asteraceae family with 16 species (22.78%), Lamiaceae with 12 sp (17.14%), Fabaceae with 6 sp (8.57%), Poaceae with 6 sp (8.57%) and Brassicaceae with 5 sp (7.14%), respectively were the must dominant families which more belongs to hemicryptophytes (51.43%) and therophytes (34.29%) life forms, respectively.

Eryngium caucasicum Trauve. belongs to Apiaceae family is one of the most medicine vegetable in traditional medicine in North of Iran. In many field observation duo to investigate of aut ecology, ethnopharmacology and antioxidant activity of Eryngium caucasicum Trauve. aerial parts of plant in blooming were collected from Larim region in sea level (1391). Ethno pharmacological data were obtained from rural healers and biological spectrum of medicinal plants obtained by Zohary method and antioxidant activity was measured by DPPH method. Results showed that this natural plant was wild grew in sandy loam soil, Ec=10.5, PH=6.63 in meditranean climate. Plant flowers appeared in Jun and falled in late of September. Biological spectrum was showed that 43 medicine plants were grow wild in this region belongs to Fabaceae (7 spp), Asteraceae (6 spp) and Lmiaceae (4 spp) and the therophytes (41.8%) were the most life form which more belongs to ES region (39.53%). the plant extract has good antioxidant activity (IC50=177.3 mg/ml) in scavenging of DPPH free radicles, so this data had confirmed the traditional uses of E.caucasicum in this region as a good spring tonic, anti-spasm, anti-inflammation and nutritive in treat of hypertension, rheumatic pain, stomatchache and expel worm.

Elaeagnus angustifolia L. is the important medicine and fruit in Iran, which possibly has different quality and quantity metabolites in different climates. The leaves, flowers and fruit of Elaeagnus angustifolia L. were collected in different region in Dargaz region (Razavi Khorasan province). Total phenol (TP), total flavonoids (TF) and total anthocyanin (TA) were obtained by spectrophotometry and anti oxidant activity of methanolic extract were measured by RP, TAC and DPPH methods. Results showed that phytochemical of extracts of plant parts were different and so plant part extracts of Ghorakh khiz region have more content of TF, TP and TA, in addition have the better anti oxidant activity in salin regions with Ec=4.6.

This research was conducted to determine of the allelopathic effect of Artemisia aucheri Boiss. extract on germination and primary growth of Amygdalus scoparia in complete randomly design with 6 treatments involved of the water extracts of aerial parts of Artemisia aucheri Boiss. as concentrations of 0 (control), 15, 30, 45, 60 and 75 gram / liter in three replications and then analyzed by multi-dimensional Duncan test. Results showed that there is a meaningful difference between the average of Amyghalus scoparia features in all the applied treatments (a=0.05), due to the length of root and the dry weight of stem are being effected and been variables in concentration of 15 gr/liter. while the germination percent, the length stem, wet weight of the stem, wet weight of the root and dry weight of stem were varied in concentration of 30 gr/liter, but the speed of germination and the number of leaf were obtained in concentration of 45 gr/liter. Data showed that with increasing of plant extract concentration, especially in 60 and 70 g/l have beter effect on plant morphological futures than 15 g/li in comparison to the evidence.

Prunus spinosa L. and Mespilus germanica L. are belonging to Rosaceae family, which have been used in traditional medicine as tonic antiseptic and anti-inflammation. In this study the leaves, flowers and fruit of both plants were collected from (Garmabdasht -700 m and Ziarat village -1215 m) randomly in Golestan province and the effect of height content on total phenol and total anthocyanin changes were measured in 4 development maturity stages of fruit (immature, mature, ripe, over ripe). Total phenol content were determined by Folin-ciocalteu reagent and total anthocyanin content by pH difference method in Spectrophotometery in 3 replicated. Results showed that amount of total phenol in blackthorn leaves are higher than M.germanica fruits and the total phenol content in its fruits reduced in during maturity periods constantly. Comparison of data mean by ANOVA in 5% level (P<0.05) showed that height factor in all cases (except the total phenol content in M.germanica immature fruits) had significant effects.

The present study conducted to evaluation of anti oxidant and phenolic content of aqueous and hydro alcoholic extracts of Ferula assa-foetida L. Plant was collected from Birjand located in south Khorasan province (1470m). Aqueous and hydro alcoholic extracts of plant were obtained by maceration method, total phenolic content was measured by folin sicalto in spectrophotometery and anti oxidant activity by scavenging free radicals of DPPH in spectrophotometry. Results showed that the hydro alcoholic extract with high phenolic content (228 mg GAE/g) and with IC50=0.415mg/ml have more anti oxidant activity than aqueous extract to free radical scavenging.