Introduction: The use of religious beliefs and spirituality as a constructive strategy in improving the psychological health of individuals. The purpose of this study was to investigate the relationship between spiritual health and depression, anxiety and stress in pregnant women. Methods: In this study, 385 pregnant women were studied. The research tool was the questionnaire "Paulotzin & Ellison Spiritual Health" and "Dass-21 Depression, Anxiety and Stress Questionnaire". Data were analyzed by SPSS software. Results: Of the 372 answered questionnaires, most had a diploma degree (1 person = 0. 8%),most were women,1 female (2 = 6. 5%) primiparous,3 months pregnant,most women Three patients (3. 9%) were in the third trimester of pregnancy. Of the pregnant women studied, 2 (6. 5%) had one or more abortions,3 (2. 9%) had no history of stillbirth and 2 (6. 5%) had a history of stillbirth. The mean "spiritual health" of pregnant women was 88. 59 16 16. 39 at the "average" level. "Depression" with 10. 57 9 9. 3 at "mild" level, "Anxiety" with 11. 66 9 9. 1 at "moderate" level and "Stress" with 13. 35 8 8. 5 at "level" Was normal. There was a significant negative correlation between spiritual health and depression, anxiety and stress in pregnant women. Conclusion: Spiritual health affects depression, anxiety, and stress during pregnancy, so proper planning is needed to prevent or reduce depression, anxiety, and stress during pregnancy.