Journal of Shahrekord University of Medical Sciences
Year:2016 | Volume:17 | Issue:5|Papers Count:13

Background and aims: Suicide in students is one of the most unfortunate events. Suicidal thoughts as self-reported thoughts are identified from a range of a vague willing for men to a total Map of suicide. One of the main methods of suicide prevention is to identify people who have thoughts of suicide One of the most important ways to prevent suicide is to identify individuals who have suicidal thoughts. The aim of this study was to investigate the prevalence rate of suicidal thoughts and its related factors among the students of Isfahan University of Medical Sciences, 2014-2015.Methods: In this cross- sectional study, 1000 students from Isfahan University of Medical Sciences were selected stratified randomly. The prevalence of suicidal thoughts and its related factors among the students were determined using Inter-cultural and SF36 questionnaires. Data were analyzed using K2 and independent t- test and logistic regression.Results: The lifetime, annual, and point prevalence of suicidal ideation among college students was 29.7%, 15% and 6.2%, respectively. A significant relationship was observed between age, gender, marital status, residential area, level of religious obligations, public health, attitudes toward suicide, justification of suicidal thoughts, and having suicidal thoughts (P<0.05). Suicidal thoughts among men and single students were more than these thoughts in women and marital students.Conclusion: This study indicated a high prevalence rate of suicidal thoughts among the university students. Regarding to suicide thoughts can produce a background for suicide procedures, so, it could prevent by representing optimal services of mental health and regular periodical screening about these thoughts and mental disorders to reduce this social problem among students.

Background and aims: Many studies have performed to find new drugs with activities against Leishmania including chemical and herbal drugs. The aim of this study was to investigate the cytotoxic effects of essential oils of Artemisia Sieberi and Pelargonium roseum against L. major promastigotes compared with Glucantime.Methods: In this experimental study, concentrations of 0.1, 0.2, 0.4, 0.8, 1.2, 1.4, 1.6, and 2 percent of both essences and concentration of 33.8 percent of Glucantime were added to the culture medium included 106 of L. major. The number of viable parasites after adding oils and Glucantime drug at 24, 48 and 72 hours was counted. Each test was repeated four times to ensure repeatability. Data were analyzed using SAS software and Tukey test.Results: The number of viable parasites after adding the herbal essences significantly decreased in comparison with culture medium included Glucantime (P£0.05). 72 hours after exposure with glucantime and herbal essences to parasite, the number of parasites decreased from 1.3×106 parasite per ml in control group to 6.06× 105 parasite per ml in treated group with glucantime and 1.6×105 and 1.7×105 in groups treated with essences of Pelargonium and Artemisia, respectively.Conclusion: Because of phylogenetic similarity between promastigot and amastigot forms of leishmania, effect of Artemisia sieberi and Pelargonium roseum oils on the amastigot of parasite is possible and use of such oils for treatment and control of amastigot form of parasite is suggested. More study is needed to verify and use this biomedical agent as an alternative potential instead of chemicals. The results of this study showed that despite the increasing resistance of promastigotes of Leishmania to chemical drugs, essential oils of plant origin can be used as an alternative therapy for the treatment of CL and the essential oils have potential effect to inhibit the proliferation of Leishmania parasites.

Background and aims: The extensive applications of nanomaterials in industry, medicine and consuming products have increased concerns about their potential toxicity. The aim of the present study was to evaluate the cytotoxic effects of copper oxide (CuO) nanopaticles on embryonic development of pregnant mice (NMRI).Methods: In this experimental study, 42 female NMRI mice were randomly divided into 6 groups and seven in each group. Four experimental groups on gestation days (GDs) 3 and 12 received CuO nanoparticle at concentrations of 300, 400, 500, 600 mg/kg intraperitoneal injection. Sham group received deionized water as CuO nanoparticle solvent and control group received no CuO nanoparticles. Mice were dissected on GD17 and embryos were separated from the uterine tissue. Histopathological studies were carried out using hematoxilin-eosin staining and light microscopy. Data were analyzed using ANOVA and Duncan test with SPSS16 software at significant level of P<0.05.Results: Findings of the present study showed that CuO nanopaticles at concentration of 600 mg/kg affect on organogenesis, complement of neural structure, liver lobulation and delay in fetal growth. Also, the average number of somites at concentrations of 400, 500 and 600 mg/kg reduced significantly compared to the control group (P<0.05).Conclusion: Damages caused by CuO nanoparticles may potentially lead to the deformity or developmental retardation of the fetus in pregnant mice.

Background and aims: The sea cucumbers were considered as an important component of aquatic ecosystems in more marine ecosystems. Melanoma as the most dangerous form of skin cancer, has long been influenced the human health. The present study was aimed to examine the cytotoxic effect of sea cucumber body wall extract, species of Holothuria arenicola on melanoma cancer cells.Methods: In this in vitro experimental study, melanoma cancer cells were cultured in 10% RPMI 1640 (Roswell Park Memorial Institute) medium and incubated at 37oC with 5% CO2. After 24 h, the cells were treated by sea cucumber body wall extract with different concentrations (15, 31, 62, 125, 250, 500 mg/ml). The cytotoxic effect of sea cucumber body wall extract were examined by using MTT assay, Acridine Orange/ Propodium Iodide, DAPI (4, 6- Diamidino-2-phenylindole) staining, flow cytometry and caspase 3, 9 assay on melanoma cancer cells. Data were analyzed using SPSS software, one way ANOVA and Duncan test.Results: Sea cucumber body wall extract induced cytotoxic effect through apoptosis induction on B16F10 melanoma cancer cells after 24 h treatment with IC50 (Inhibitory Concentration)= 31 mg/ml significantly(P<0.05). This extract were exerted more toxicity in concentration of 62 mg/ml as a result around 80% of the cells were apoptotic.Conclusion: Findings showed that the sea cucumber body wall extract with cytotoxic effect and pro apoptosis promoter in B16F10 melanoma cell line can be a suitable candidate for anti-cancer researches.

Background and aims: Patients’ satisfaction is an important indicator to evaluate and measure the efficiency and quality of services provided to people in the health care system and treatment is one of the main priorities in the Ministry of Health. So, the aim of this study was to evaluate the effect of automated telephone system on the client satisfaction of Imam Ali polyclinic.Methods: This analytic cross- sectional study was conducted in Shahrekord Imam Ali specialty and subspecialty polyclinic in 2013. The standard satisfaction questionnaire was completed with participation of 700 patients. Data were analyzed using descriptive and inferential statistics with using SPSS 16.Results: The mean score of patients’ satisfaction in 2012 was 22.5±5.7 and in 2013 was 23.7±6.3, whereas differences in 2 years indicate statistically significant in satisfaction of clients (P=0.002). Turning system indicators, the turning process, waiting room, clinic cleanliness, guards behaviors, acceptance and secretary behaviors, physician visit, privacy respecting and choice of clinic for a follow up visit, explained 78% of the total patients’ satisfaction.Conclusion: Modification of processes of admitting such as turning is caused to increase patients’ satisfaction. So, regular and periodic evaluation of patients’ satisfaction and providing operational programs to reduce their unsatisfaction can lead to improving the quality of health services.

Background and aims: Carbamazepine is one of the most effective medicines in treating epilepsy; and its use is associated with teratogenic effects. The aim of this study was to evaluate the effect of carbamazepine during gonad development in female rats.Methods: In this experimental study, 60 female pregnant rats were divided into 2 classes (n=8). Each class contains control, sham with doses 10, 15, 30 mg/kg of carbamazepine. All intraperitoneal injections were performed during 14-18 days of pregnant (for fetal gonad development) and 0-10 days after birth (for newborn gonad development). The newborns were allowed to reach 7 weeks old. The right ovary was removed and histological staining (Hematoxylin and Eosin) was performed. Data were analyzed using one and two ANOVA, and Tukey test.Results: The results showed that carbamazepine causes increasing in the number of primary oocyte, but decreasing the number of secondary oocyte, graffian follicles and corpus luteum. It showed that the number of primary oocyte in fetal treated group with 30 mg/kg of carbamazepine has a significant increase compared to the fetal and newborn development rats groups (P<0.001). The number of secondary oocyte in all fetal treated groups showed a significant decrease compared to the other group (P<0.01). Also, it showed that the number of graffian follicles and corpus luteum in fetal treated groups has a significant decrease in comparison to the newborn treated rats (P<0.001).Conclusion: This study showed that carbamazepine can remain negative effects on female sex glands during organogenesis processes. So, it seems that gonads are very sensitive to carbamazepine and its sensitivity is dependent to different doses.

Background and aims: Recently, it has been paid attention to silver nanoparticles (SNPs), but less attention has been to their toxicity. This study was aimed to investigate acute effects of oral silver nanoparticles (SNPs) on biochemical parameters of kidney (Creatinine and BUN) and blood electrolytes in laboratory male mice (Mus musculus).Methods: In this experimental study, A group 45 BALB/c mice about 4 weeks (weighting 24.2±3.0 g) were randomly divided into three groups: 2 nanosilver (SNPs) treatments (1,2) and one control group, each with 15 mice. Once a day at the same time, a volume of 50 microliters from the nanosilver solution (20 and 50 ppm) was administered to the treatment groups while in the untreated (control) group only distilled water was used. Then, on 2, 7, and 14 day, blood sampling was done using cardiac puncture. Serum level of creatinine, BUN, sodium and potassium were measured in the control and treatments (20ppm-treated group and 50ppm-treated group). Data were analyzed using SPSS software and two way ANOVA test at significant level of P<0.05.Results: On day 2 of the experiment, average level of creatinine of 20ppm-treated group was significantly higher than to the control group (P>0.05), but levels of blood urea nitrogen in both 20- and 50ppm-treated groups compared to the control were not significant(P>0.05). In addition, average sodium level of 20ppm-treated group showed a significant increase in relation to the control group on day 14 (P<0.05). Average potassium level of 50ppm-treated group showed a significant decrease in relation to the control group on day 2 (P<0.05). Moreover, average potassium level in 20 ppm-treated group on day 7 and in both 20- and 50ppm-treated groups on day 14 decreased significantly (P<0.05).Conclusion: This study showed that orally-administered SNPs may have negative effects on the biochemical parameters of kidney and blood electrolytes. They should be applied cautiously.

Background and aims: Thermal oxidation of oils is a decomposition process in which hydroperoxide is produced. Free radicals are formed by the decomposition of hydro peroxides that have cytotoxic and genotoxic nature. Peroxide value is an index by which to determine the extent of the oxidation of fats and oils. The aim of this study was to determine peroxide value and visual color of Zoolbia and Bamieh oils in the holy month of Ramadan.Methods: This research was a descriptive cross-sectional study. In this study, a total of 222 oil samples used for the production Zoolbia and Bamieh were collected randomly by environment health inspectors of healthcare networks from small manufacturing units in Chaharmahal and Bakhtiari province during holy month of Ramadan in 2014. These samples were sent for healthy control to the laboratory of food materials in Shahrekourd University of Medical Sciences. Color of samples was checked apparently, and the rate of peroxides in samples was determined. Data were analyzed using descriptive tests and SPSS software.Results: This study showed that peroxide value of 9.5% (22 of 222) of the tested oil samples was higher than national standard maximum limit of edible oils (5 mEq/kg). The average peroxide value in these oil samples was 2.95 mEq/kg. In total, the visual color of 36.9% (82 of 222) of oil samples was dark to black.Conclusion: The above results show that more than one- third of Zoolbia and Bamieh oils are not safe and immune and they threaten the health of consumers. Therefore, training and inspection for producers these kinds of foods materials are essential and important.

Background and aims: Most people with lateral ankle sprain don’t recover completely and imbalance is one of the most common problems that occur in the damaged persons. Regarding to Athletic Kinesio Taping was introduced as a new therapeutic approach and has been used in sport medicine; this study was aimed to investigate the effectiveness of Kinesio Taping on dynamic balance in athletes with acute sprain and chronic ankles instable.Methods: In this quasi-experimental study, 26 men athletes (14 individuals with chronic instability; age mean 21.4±2.1, BMI 22.3±3.1 and 12 cases with acute sprain; age 20.7±2.3, BMI 22.4±2.7) in normal conditions were evaluated with Star Excursion Balance Test (SEBT) in 2 positions untapped and taped with mechanical-functional correction Kinesio Taping method. Data were analyzed using t- test at significant level of (P£0.05).Results: Quantities difference between pretest and posttest of SEBT was significant in both groups and ankle Kinesio Taping has affected on dynamic balance. Moreover, differences were more in group with acute injury (P£0.05).Conclusion: Mechanical-functional crrection Kinesio Taping method improves dynamic balance in athletes with acute sprain and chronic ankles instable immediately and Kinesio Taping showed a better result in balance of acute sprain group.

Background and aims: Regret after vasectomy affects relations between couples besides imposing costs for reopening vases. The aim of this study was to investigate the regret rate in vasectomized individuals and its reasons, which can help the health planners to improve the situation.Method: This cross-sectional study investigated 157 vasectomized men by average 5 years old from 2000 to 2010 years in Shahrekord town. Samples were selected randomly through referring to the patients’ medical files in Kashani Hospital in Shahrekord, and visiting them at home’s door. Data were collected and analyzed using SPSS software and K2 and descriptive statistical tests.Results: In this study, 15.9% of the individuals expressed regret from vasectomy. The most important reasons were the incidence of sexual side effects (40%) and complications of surgery (28%). Also, people who had vasectomy because of poor economic condition regretted after vasectomy more than others. In this study, in cases where health workers were main advocates of vasectomy, the highest satisfaction with vasectomy was obtained. The individuals who developed post-vasectomy complications with long term complications regretted after vasectomy more than others.Conclusion: In view of findings in this study and role of post-vasectomy complications in the regret rate in the individuals and the fact they are not consulted after vasectomy, it is recommended counseling and interventional programs with acceptable quality and specialized workforce after vasectomy.

Background and aims: Todays, the use of substances secreted by some probiotic bacteria as inhibitor and antibacterial substance is in high importance. The aim of this study was to evaluate of antimicrobial activity of Lactobacillus plantarum and ruteri in Acinetobacter baumannii isolated from nosocomial infections.Methods: In this experimental study, it was investigated Acinetobacter isolated of 100 patients hospitalized in Milad, Daneshvari and Mofid hospitals in Tehran. Antibiotic resistance of Acinetobacter with 2 strains of Lactobacillus plantarum and ruteri was determined. The inhibitory activity of 2 Lactobacillus plantarum and ruteri against Acinetobacter baumannii were determined by using supernatant solution of 48 hours culture of lactobacilli in 2 forms of active and passive according agar well diffusion method.Results: Results showed that Lactobacillus plantarum and ruteri were sensitive to vancomycin, imipenem, ciproflaxin, ceftazidime and resistance to piperacillin, colistin. Cotrimoxazol. Acinetobacter baumannii was also resistance to vancomycin, cortrimoxazol, piperacillin, ceftazidine, and sensitive to ciproflaxin and imipenem. Supernatant solution of lactobacillus plantarum and ruteri cultur has had more antibacterial activity in presence of hydrogen peroxide and natural pH compared to non-active solution.Conclusion: The results of this study showed that Lactobacillus plantarum and ruteri hadn’t antibacterial activity against Acinetobacter baumannii in inactive condition, but the culture supernatant lactobacilli had significant activity against strains of hospital pathogens under conditions of acid and hydrogen peroxide solution. Moreover, 2 Lactobacillius (plantarum and ruteri) were nearly resistance to common antibiotics.

Background and aims: The reduction in blood flow may result pathological changes in testicular and spermatogenesis function. Moreover, free iron ions result of lysing red blood cells and consequently the increase in iron ions content of testes leads to oxidative stress with reproductive system dysfunction. So, the present study was aimed to evaluate the protective effect of the simultaneous use of royal jelly (RJ) and vitamin C (Vit C) on hemolytic anemia condition along with decomposition of red blood cells.Methods: In this experimental study, 32 mature mice weighting 20-25 gr were divide into 4 groups including control group received normal saline (0.2 mL, IP) with intraperitoneal injection, sham control group administered with Phenylhydrazine (PHZ) (6 mg/100g , IP), PHZ+ Vit C with (250 mg/kg, IP)+ RJ (100 mg/kg,) group orally and RJ+Vit C group. After 35 days, blood samples were obtained from heart with the evaluation of serum levels of total antioxidant capacity (TAC) and malondialdehyde (MDA). Also, the sperm quality including sperm motility, viability, sperm count, chromatin condensation and DNA damage was assessed.Results: There was a significant reduction in TAC level of PHZ group in the present study, and co-administrating Vit C and RJ in receiver group of antioxidants could prevent this decrease. Moreover, the PHZ caused the serum level of MDA is increased. Meanwhile, co-administrating the Vit C and RJ diminished MDA in PHZ-administrated animals. Finally, co-administrating the Vit C and RJ by scavengering of free radicals resulted in remarkable enhancement in quality of sperm parameters (P<0.05).Conclusion: PHZ can disorder sperm parameters through inducing oxidative stress. Co-administrating of the Vit C and RJ may be significantly up-regulated the serum TAC level and down regulating of MDA promoted the sperm quality.