Acute toxicity and effects of diazinon on some hematological parameters of Acipenser guldenstadti of 12±2 g body weight were studied under static water quality conditions at 22±10c. The values of 6.15, 5.15, 5.11 and 5.09 mg/L were calculated as LC50 after 24, 48, 72 and 96 hours, respectively, while those of LC50 were calculated 7.21, 6.70, 6.13 and 6.09 mg/L, respectively. The affected fish were paralysed showing restless, cramp movements, loss of coordination and orientation in water column. Fish then turned on the flank and swam in half-circles followed by sudden movements and fin tremor. Also, darkening of dorsal body surface and block of respiratory movements were observed before fish death in a short time. After 96 hours exposing fish of 12 g body weight to LC50 concentration of the toxicant (6.09 mg/L), hematological indices of erythrocyte (RBC), haematocrit (PCV), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), leukocyte (WBC) and lymphocyte counts were significantly lower than control (P<0.05), while there was a significant increase in values of neutrophiles and monocytes of test group compared to control one (P<0.05). Also, there was no significant difference in the levels of mean corpuscular hemoglobin concentration (MCHC), leucocryte, eosinophile and immature neutrophile bewteen test and control groups (P>0.05). Also, the levels of alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and total protein were significantly lower in fish exposed to the toxicant compared to control fish (P<0.05), while level of glucose was significantly higher in experimental fish (P<0.05).