In this research, physicochemical and sensory properties of ice cream by substitution of Milk Solids Non Fat (MSNF) with different levels of Whey Protein Concentrate (WPC) in ice cream mix formulation were evaluated. Two kinds of WPC produced by two different methods, precipitation by carboxymethylcellulose (CMC-WPC) and concentration by ultrafiltration (UF-WPC), at three substitution levels of 0, 25 and 50, two kinds of stabilizer (salep and palsgard) and also two aging times (4 and 6 hours) were considered as research variables. All mixes were formulated to make an ice cream containing fat 5%, MSNF 10%, sugar 18% and stabilizer 0.4%. Vanilla and rose water were added to all of the formulations to improve their taste and aroma. Physicochemical properties of different samples of ice cream treated as above including overrun, melting resistance, viscosity, density and pH were measured and the results were analyzed as a factorial experiment in completely randomized design. Sensory properties of samples (taste, flavor, texture and total acceptance) were judged by 10 panelists in five points hedonic test scale and the results were compared with control ice cream (without MSNF substitution). Data analysis showed that the kind of WPC had no effect on pH, density and sensory properties of ice cream samples, but viscosity, overrun and melting resistance were significantly affected. Samples containing CMC-WPC had higher viscosity and melting resistance and lower overrun than samples containing UF-WPC. All of the physical and sensory properties (except aroma score) were significantly affected by different levels of WPC substitute. In comparison with control formulation, overrun, melting resistance, pH and all of the sensory properties points (except aroma score) were decreased and viscosity and density were increased as the level of WPC replacement was increased. Decrease in sensory properties scores was not significant at 25% replacement level, but it became significant at 50% replacement level.