The Cytotoxic Effects of Hydroalcoholic Extract of Aerial Parts of Scrophularia striata on MCF-7 Cell Line and Evaluation of BAX and KAI1 Genes Expression

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Tabatabaeinejad Modaddeseh Sadat; AHMADI RAHIM; KASSAEE SEYED MEHRDAD

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Journal: Qom University of Medical Sciences Journal Year:2018 | Volume:12 | Issue:9 Page(s): 38-46

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Title

The Cytotoxic Effects of Hydroalcoholic Extract of Aerial Parts of Scrophularia striata on MCF-7 Cell Line and Evaluation of BAX and KAI1 Genes Expression

Author(s)

Tabatabaeinejad Modaddeseh Sadat | AHMADI RAHIM | KASSAEE SEYED MEHRDAD | Issue Writer Certificate

Keywords

MCF-7 CellsQ1

Schrophularia striataQ1

Human BAXQ1

KAI1Q1

Abstract

Background and Objectives: Studies have shown that Scrophularia striata has various treatment effects. For this purpose, in this study, the cytotoxic effect of hydroalcoholic extract of aerial parts of Scrophularia striata was investigated on MCF-7 cell line, and BAX and KAI1 genes expression was assessed. Methods: In this laboratory experimental study, at first, extract of the plant was prepared using maceration method. The effect of different concentrations of the extract (0. 001, 0. 001, 0. 1, 1, and 10mg/ml) was investigated on the viability of MCF-7 Cells using MTT assay method. Also, the effects of doses of 1, 0. 1, and 0. 01mg/ml of the extract, was evaluated on BAX and KAI1 genes using real-time PCR. The data were analyzed using one way AN0VA test. Results: . In this study, all concentrations of the extract caused a significant decrease in viability of MCF7 cells compared to the control group (p<0. 001). On the one hand, the relative expression level of antimetastasis KAI1 gene and BAX apoptotic gene significantly decreased in the groups that received 0. 1 and 1 mg/ml of extract compared to the control group (p<0. 001). Conclusion: The findings of this study showed that hydroalcoholic extract of aerial parts of Scrophularia striata has cytotoxic effects on breast cancer cells, which, in this regard, can induce of BAX gene dependent apoptosis. Moreover, this extract has antimetastatic effects on breast cancer cells through increasing the relative expression level of antimetastasis KAI1 gene.

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APA: Copy

Tabatabaeinejad, Modaddeseh Sadat, AHMADI, RAHIM, & KASSAEE, SEYED MEHRDAD. (2018). The Cytotoxic Effects of Hydroalcoholic Extract of Aerial Parts of Scrophularia striata on MCF-7 Cell Line and Evaluation of BAX and KAI1 Genes Expression. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, 12(9 ), 38-46. SID. https://sid.ir/paper/132078/en

Vancouver: Copy

Tabatabaeinejad Modaddeseh Sadat, AHMADI RAHIM, KASSAEE SEYED MEHRDAD. The Cytotoxic Effects of Hydroalcoholic Extract of Aerial Parts of Scrophularia striata on MCF-7 Cell Line and Evaluation of BAX and KAI1 Genes Expression. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL[Internet]. 2018;12(9 ):38-46. Available from: https://sid.ir/paper/132078/en

IEEE: Copy

Modaddeseh Sadat Tabatabaeinejad, RAHIM AHMADI, and SEYED MEHRDAD KASSAEE, “The Cytotoxic Effects of Hydroalcoholic Extract of Aerial Parts of Scrophularia striata on MCF-7 Cell Line and Evaluation of BAX and KAI1 Genes Expression,” QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, vol. 12, no. 9 , pp. 38–46, 2018, [Online]. Available: https://sid.ir/paper/132078/en

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