ENRICHMENT OF INTACT PERIPHERAL BLOOD DENDRITIC CELLS BY IMMUNOMAGNETIC DEPLETION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NADERI N.; POURFATH ELAH A.A.; ALI MOGHADAM K.; GHAVAMZADEH ARDESHIR; MOAZENI S.M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2004 | Volume:6 | Issue:22 Page(s): 91-96

Download Full-Text

Persian Verion

View:

1,222

Download:

Cites:

Information Journal Paper

Title

ENRICHMENT OF INTACT PERIPHERAL BLOOD DENDRITIC CELLS BY IMMUNOMAGNETIC DEPLETION

Author(s)

Keywords

PERIPHERAL BLOOD DENDRITC CELLSQ3

NEGATIVE SELECTIONQ3

IMMUNOMAGNETIC BEADSQ3

Abstract

Introduction: Enrichment of intact peripheral blood dendritic cells (DCs) has been done using rosette and immunomagnetic depletion techniques. Material and Methods: Peripheral blood mononuclear cells were separated using ficoll density gradient centrifugation and T lymphocytes were depleted through reaction with AET- treated sheep red blood cells. The remaining cells were depleted from lineage marker positive cells using monoclonal antibodies against CD3, CD11b, CD14, CD16, CD19, CD56 and anti- mouse IgG magnetic beads. Flowcytometric analysis was used to determine the purity of the obtained DCs through HLA-DR expression and lack of lineage markers. Results: The obtained results showed that a significant population of isolated cells (58.5% ± 4.5, n=5) fail to react with anti-lineage marker monoclonal antibodies but express the HLA-DR antigen. Conclusion Immunomagnetic depletion is a new method for DCs separation that unlike classic methods, can isolate DCs in intact form. According to our information, this is the first report of DCs separation in Iran and purity of the obtained DCs is comparable with similar studies in other countries. Significant enrichment of intact DCs could facilitate the study of DCs natural functions in different fields such as autoimmunity, cancers and infectious diseases.

Cites

No record.

References

Cite

APA: Copy

NADERI, N., POURFATH ELAH, A.A., ALI MOGHADAM, K., GHAVAMZADEH, ARDESHIR, & MOAZENI, S.M.. (2004). ENRICHMENT OF INTACT PERIPHERAL BLOOD DENDRITIC CELLS BY IMMUNOMAGNETIC DEPLETION. CELL JOURNAL (YAKHTEH), 6(22), 91-96. SID. https://sid.ir/paper/16437/en

Vancouver: Copy

NADERI N., POURFATH ELAH A.A., ALI MOGHADAM K., GHAVAMZADEH ARDESHIR, MOAZENI S.M.. ENRICHMENT OF INTACT PERIPHERAL BLOOD DENDRITIC CELLS BY IMMUNOMAGNETIC DEPLETION. CELL JOURNAL (YAKHTEH)[Internet]. 2004;6(22):91-96. Available from: https://sid.ir/paper/16437/en

IEEE: Copy

N. NADERI, A.A. POURFATH ELAH, K. ALI MOGHADAM, ARDESHIR GHAVAMZADEH, and S.M. MOAZENI, “ENRICHMENT OF INTACT PERIPHERAL BLOOD DENDRITIC CELLS BY IMMUNOMAGNETIC DEPLETION,” CELL JOURNAL (YAKHTEH), vol. 6, no. 22, pp. 91–96, 2004, [Online]. Available: https://sid.ir/paper/16437/en

Related Journal Papers