REACTION OF NATIVE AND DENATURED BRUCELLA ABORTUS (S19) PROTEINS WITH ANTIBODY USING AFFINITY CHROMATOGRAPHY AND IMMUNOBLOTTING

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KARIMI R.; MOSTAFAIE A.; TABARAEI BAHMAN; BAHRAMI Y.; ABD ALALIZADEH J.

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Journal: Avicenna Journal of Clinical Medicine Year:2005 | Volume:11 | Issue:4 (SN 34) Page(s): 14-22

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Title

REACTION OF NATIVE AND DENATURED BRUCELLA ABORTUS (S19) PROTEINS WITH ANTIBODY USING AFFINITY CHROMATOGRAPHY AND IMMUNOBLOTTING

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Keywords

BRUCELLA ABORTUSQ3

CHROMATOGRAPHYQ3

AFFINITYQ3

IMMUNOBLOTTINGQ3

Abstract

Western blotting or IMMUNOBLOTTING commonly use for study of reaction between antigens and antibodies. Denaturation of many proteins in IMMUNOBLOTTING can affect greatly the reactivity of antibodies and outcome of the procedure. In this study proteins of BRUCELLA ABORTUS (S19) was extracted by a mild method and reaction of the extracted proteins with serum of infected human and goat and immunized rabbit compared by AFFINITY CHROMATOGRAPHY and IMMUNOBLOTTING. Gamma globulin (mostly IgG) fraction of the sera was precipitated by half saturation of ammonium sulfate and linked to activated sepharose 4B. The extracted proteins were loaded on the AFFINITY column. Attached proteins was eluted by low pH and analyzed by SDS-PAGE. Reaction of the total extract and eluted fractions with IgG fraction of sera was evaluated by Western blotting.Upon the results of AFFINITY CHROMATOGRAPHY and IMMUNOBLOTTING, Brucella proteins can be classified in four groups: 1- The proteins that adsorbed to the AFFINITY column and react with IgG in westernblotting. 2- Proteins that react with IgG in native state but no in denatured state. 3- Proteins that do not react with IgG in native state but react in denatured state. 4- Proteins that do not react with IgG in native and denatured state.

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APA: Copy

KARIMI, R., MOSTAFAIE, A., TABARAEI, BAHMAN, BAHRAMI, Y., & ABD ALALIZADEH, J.. (2005). REACTION OF NATIVE AND DENATURED BRUCELLA ABORTUS (S19) PROTEINS WITH ANTIBODY USING AFFINITY CHROMATOGRAPHY AND IMMUNOBLOTTING. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 11(4 (SN 34)), 14-22. SID. https://sid.ir/paper/17359/en

Vancouver: Copy

KARIMI R., MOSTAFAIE A., TABARAEI BAHMAN, BAHRAMI Y., ABD ALALIZADEH J.. REACTION OF NATIVE AND DENATURED BRUCELLA ABORTUS (S19) PROTEINS WITH ANTIBODY USING AFFINITY CHROMATOGRAPHY AND IMMUNOBLOTTING. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2005;11(4 (SN 34)):14-22. Available from: https://sid.ir/paper/17359/en

IEEE: Copy

R. KARIMI, A. MOSTAFAIE, BAHMAN TABARAEI, Y. BAHRAMI, and J. ABD ALALIZADEH, “REACTION OF NATIVE AND DENATURED BRUCELLA ABORTUS (S19) PROTEINS WITH ANTIBODY USING AFFINITY CHROMATOGRAPHY AND IMMUNOBLOTTING,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 11, no. 4 (SN 34), pp. 14–22, 2005, [Online]. Available: https://sid.ir/paper/17359/en

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