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Information Journal Paper

Title

HUMAN OVARIAN TISSUE VITRIFICATION/WARMING HAS MINOR EFFECT ON THE EXPRESSION OF APOPTOSIS-RELATED GENES

Pages

  179-186

Abstract

 Background: In this study, we evaluated the incidence of APOPTOSIS at the ultrastructural levels and expression of some APOPTOSIS-related genes in vitrified human ovarian tissue just after warming.Methods: Human ovarian tissue biopsies from 23 women after caesarean section were transported to the laboratory within 2 hours, and then they were cut into small pieces. Some pieces were vitrified and warmed and the other samples were considered as control. APOPTOSIS was assessed by a transmission electron microscope and also by molecular analysis of proapoptotic (Fas, FasL, Bax, p53, caspase8, and caspase3) and antiapoptotic (Bcl-2 and BIRC5) genem RNA levels using real-time RT-PCR before and after VITRIFICATION.Results: No sign of APOPTOSIS was shown ultrastructurally in vitrified samples. The level of FasL, Bcl-2, Bax, p53, and caspase3 mRNA and Bax: Bcl-2 ratio were similar in non-vitrified and vitrified groups; however, the expression of Fas and caspase8 genes was higher and BIRC5 was lower in vitrified samples compared to non-vitrified group (P<0.05).Conclusion: The fine structure of human vitrified ovarian tissue was well preserved; moreover, VITRIFICATION was shown to affect the expression of some APOPTOSIS-related genes. However, additional study is needed to confirm this observation.

Cites

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    APA: Copy

    ABDOLLAHI, MASOUME, SALEHNIA, MOJDEH, SALEHPOUR, SAGHAR, & GHORBANMEHR, NASSIM. (2013). HUMAN OVARIAN TISSUE VITRIFICATION/WARMING HAS MINOR EFFECT ON THE EXPRESSION OF APOPTOSIS-RELATED GENES. IRANIAN BIOMEDICAL JOURNAL, 17(4), 179-186. SID. https://sid.ir/paper/277364/en

    Vancouver: Copy

    ABDOLLAHI MASOUME, SALEHNIA MOJDEH, SALEHPOUR SAGHAR, GHORBANMEHR NASSIM. HUMAN OVARIAN TISSUE VITRIFICATION/WARMING HAS MINOR EFFECT ON THE EXPRESSION OF APOPTOSIS-RELATED GENES. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2013;17(4):179-186. Available from: https://sid.ir/paper/277364/en

    IEEE: Copy

    MASOUME ABDOLLAHI, MOJDEH SALEHNIA, SAGHAR SALEHPOUR, and NASSIM GHORBANMEHR, “HUMAN OVARIAN TISSUE VITRIFICATION/WARMING HAS MINOR EFFECT ON THE EXPRESSION OF APOPTOSIS-RELATED GENES,” IRANIAN BIOMEDICAL JOURNAL, vol. 17, no. 4, pp. 179–186, 2013, [Online]. Available: https://sid.ir/paper/277364/en

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