EVALUATION OF A SIMPLE AND RAPID METHOD FOR SERUM NITRIC OXIDE DETERMINATION USING MICROPLATE

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GHASEMI ASGHAR; HEDAYATI MAHDI; KHOUSHBATEN A.

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Journal: IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM) Year:2005 | Volume:7 | Issue:SUPPLEMENT 4 Page(s): 433-439

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Information Journal Paper

Title

EVALUATION OF A SIMPLE AND RAPID METHOD FOR SERUM NITRIC OXIDE DETERMINATION USING MICROPLATE

Author(s)

GHASEMI ASGHAR | HEDAYATI MAHDI | KHOUSHBATEN A. | Issue Writer Certificate

Keywords

NITRIC OXIDEQ2

GREISS REACTIONQ4

COLORIMETRYQ4

MICROPLATEQ4

SENSITIVITYQ3

PRECISIONQ3

ACCURACYQ4

Abstract

Introduction: Increasing interest to study the effect of NITRIC OXIDE (NO) arise the need to establish a rapid and simple method for its determination. NITRIC OXIDE has a short half life, and as a result its direct determination is difficult; hence measurement of its metabolites (nitrite and nitrate) is preferred. This assay provides a good estimation of NO output in vivo. Although NOx determination with GREISS REACTION has a long history but for determination of NOx in large numbers of samples, development, evaluation and validation of a simple, rapid and non expensive MICROPLATE format method was the aim of this study.Materials and Methods: For this purpose, nitrate was reduced to nitrite by vanadium chloride (III). Diazotization of sulfonamide by nitrous acid and its conjugation with a bicyclic amine produced a chromophor, which had max absorbance at 540 nm. The concentration of unknown samples was determined on the basis of standard curve. Results: The results showed that the standard curve was linear up to 150 mM. SENSITIVITY was 2 mM. Intra and inter assays coefficient of variations were less than 6% and the recovery was 91-93% indicating acceptable method PRECISION and ACCURACY respectively. Conclusion: In conclusion the method developed had acceptable PRECISION and ACCURACY for NO determination.

Cites

COMPARING DEPROTEINIZATION METHODS FOR SERUM NITRIC OXIDE ASSAY BY GREISS REACTION

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APA: Copy

GHASEMI, ASGHAR, HEDAYATI, MAHDI, & KHOUSHBATEN, A.. (2005). EVALUATION OF A SIMPLE AND RAPID METHOD FOR SERUM NITRIC OXIDE DETERMINATION USING MICROPLATE. IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM), 7(SUPPLEMENT 4), 433-439. SID. https://sid.ir/paper/27802/en

Vancouver: Copy

GHASEMI ASGHAR, HEDAYATI MAHDI, KHOUSHBATEN A.. EVALUATION OF A SIMPLE AND RAPID METHOD FOR SERUM NITRIC OXIDE DETERMINATION USING MICROPLATE. IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM)[Internet]. 2005;7(SUPPLEMENT 4):433-439. Available from: https://sid.ir/paper/27802/en

IEEE: Copy

ASGHAR GHASEMI, MAHDI HEDAYATI, and A. KHOUSHBATEN, “EVALUATION OF A SIMPLE AND RAPID METHOD FOR SERUM NITRIC OXIDE DETERMINATION USING MICROPLATE,” IRANIAN JOURNAL OF ENDOCRINOLOGY AND METABOLISM (IJEM), vol. 7, no. SUPPLEMENT 4, pp. 433–439, 2005, [Online]. Available: https://sid.ir/paper/27802/en

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