COMPARING DEPROTEINIZATION METHODS FOR SERUM NITRIC OXIDE ASSAY BY GREISS REACTION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHASEMI A.; HEDAYATI S.M.; BIABANI H.; KHOUSHBATEN A.; ASGARI A.R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Research in Medicine Year:2007 | Volume:31 | Issue:1 Page(s): 33-37

Download Full-Text

Persian Verion

View:

1,291

Download:

Cites:

Information Journal Paper

Title

COMPARING DEPROTEINIZATION METHODS FOR SERUM NITRIC OXIDE ASSAY BY GREISS REACTION

Author(s)

Keywords

NITRIC OXIDEQ2

DEPROTEINIZATIONQ3

GREISS REACTIONQ3

SERUMQ2

Abstract

Background: NITRIC OXIDE (NO) is involved in numerous physiologic and phathophysiologic processes. Recently, further investigators have focused on SERUM NO determination. In our previous study, we validated a simple, cheap and rapid method for SERUM NO determination based on the GREISS REACTION. DEPROTEINIZATION is a necessary step for this reaction, thus, the present study was designed to assess different DEPROTEINIZATION methods for SERUM NO determination. Materials and methods: Ten common protein precipitating chemicals including methanol, ethanol, zinc sulfate, methanol/diethyl ether, acetonitrile, TCA, PCA, sodium tangstate, ammonium sulfate and filter were used for DEPROTEINIZATION of 42 human sera, while results were compared to filter separation as a reference. SERUM NO levels were determined in 60 sera of adult human. Results: Data showed that correlation coefficient of precipitating agents: methanol, ethanol, zinc sulfate, methanol-diethylether, acetonitrile, TCA, PCA, sodium tangstate, ammonium sulfate against filter separation method were 0.84, 0.92, 0.91, 0.79, 0.88, 0.85, 0.93, 0.53, and 0.78, respectively (p<0.001).Methanol, ethanol and methanol/diethylether caused overestimation, while TCA, PCA, sodium tangstate, and ammonium sulfate caused underestimation of SERUM NO results. SERUM NO level had normal distribution with mean ±SE of 33±1.3 mmol/L. Conclusion: Although different chemical protein precipitants are used for SERUM NO determination, our study revealed that zinc sulfate is the best choice for this purpose.

Cites

EVALUATION OF THE EFFECT OF ALLIUM SATIVUM AQUEOUS EXTRACT ON SERUM NITRIC OXIDE IN MICE

References

EVALUATION OF A SIMPLE AND RAPID METHOD FOR SERUM NITRIC OXIDE DETERMINATION USING MICROPLATE

Cite

APA: Copy

GHASEMI, A., HEDAYATI, S.M., BIABANI, H., KHOUSHBATEN, A., & ASGARI, A.R.. (2007). COMPARING DEPROTEINIZATION METHODS FOR SERUM NITRIC OXIDE ASSAY BY GREISS REACTION. PAJOUHESH DAR PEZESHKI, 31(1), 33-37. SID. https://sid.ir/paper/41676/en

Vancouver: Copy

GHASEMI A., HEDAYATI S.M., BIABANI H., KHOUSHBATEN A., ASGARI A.R.. COMPARING DEPROTEINIZATION METHODS FOR SERUM NITRIC OXIDE ASSAY BY GREISS REACTION. PAJOUHESH DAR PEZESHKI[Internet]. 2007;31(1):33-37. Available from: https://sid.ir/paper/41676/en

IEEE: Copy

A. GHASEMI, S.M. HEDAYATI, H. BIABANI, A. KHOUSHBATEN, and A.R. ASGARI, “COMPARING DEPROTEINIZATION METHODS FOR SERUM NITRIC OXIDE ASSAY BY GREISS REACTION,” PAJOUHESH DAR PEZESHKI, vol. 31, no. 1, pp. 33–37, 2007, [Online]. Available: https://sid.ir/paper/41676/en

Related Journal Papers