EVALUATION OF DIFFERENTIATION POTENTIAL OF MENSTRUAL BLOOD-DERIVED STEM CELLS TO CARDIOMYOCYTES IN VITRO

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

RAHIMI MARYAM; ZARNANI AMIR HASSAN; MOHSENI KOUCHESFEHANI HOMA; KAZEMNEJAD SOMAIEH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Daneshvar Medicine: Basic and Clinical Research Journal Year:2016 | Volume:23 | Issue:123 Page(s): 31-42

Download Full-Text

Persian Verion

View:

1,230

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF DIFFERENTIATION POTENTIAL OF MENSTRUAL BLOOD-DERIVED STEM CELLS TO CARDIOMYOCYTES IN VITRO

Author(s)

RAHIMI MARYAM | ZARNANI AMIR HASSAN | MOHSENI KOUCHESFEHANI HOMA | KAZEMNEJAD SOMAIEH | Issue Writer Certificate

Keywords

MENSTRUAL BLOODQ1

STEM CELLQ2

CARDIOMYOCYTEQ2

DIFFERENTIATIONQ2

Abstract

Background and Objective: In recent decades, STEM CELL therapy has been introduced as a novel therapeutic approach for patients suffering from cardiac disorders. Recently, identification of MENSTRUAL BLOOD-derived STEM CELLs (MenSCs) as a unique source of STEM CELL with some characteristics as well as ease of access, high proliferative ability and renewability has created enormous promise for cell therapy.Materials and Methods: In this study, DIFFERENTIATION ability of MenSCs into CARDIOMYOCYTEs has been investigated. After MenSCs immunophenotyping, their DIFFERENTIATION into CARDIOMYOCYTE was investigated in the presence of 5-azacytidine and basic-fibroblast growth factor. Then, expression of the putative myogenic cells at mRNA and protein levels was determined by immunofluorescent staining and real-time quantitative PCR.Results: Based on flow cytometric analysis, the isolated MenSCs typically expressed mesenchymal STEM CELL markers like CD105, CD73, CD44 and CD166 in parallel to OCT-4 as an embryonic marker. The differentiated MenSCs expressed CARDIOMYOCYTE markers at mRNA/protein level. The myogenic cells differentiated from MenSCs were positive for Connexin-43 and troponin T2 (TNNT2) protein. The mRNAs of Connexin-43, Connexin-40, Alpha actinin, Tropomyosin1 and TNNT2 were highly expressed in the differentiated myogenic cells.Conclusion: Based on our data, MenSCs are a unique cell population with DIFFERENTIATION ability into cells with characteristics commonly attributed to CARDIOMYOCYTEs.

Cites

No record.

References

No record.

Cite

APA: Copy

. (1395). ارزیابی توان تمایز سلول های بنیادی مشتق از خون قاعدگی به سلول های قلبی در شرایط آزمایشگاهی. دانشور پزشکی, 23(123), 31-42. SID. https://sid.ir/paper/30394/en

Vancouver: Copy

. ارزیابی توان تمایز سلول های بنیادی مشتق از خون قاعدگی به سلول های قلبی در شرایط آزمایشگاهی. دانشور پزشکی[Internet]. 1395;23(123):31-42. Available from: https://sid.ir/paper/30394/en

IEEE: Copy

, “ارزیابی توان تمایز سلول های بنیادی مشتق از خون قاعدگی به سلول های قلبی در شرایط آزمایشگاهی,” دانشور پزشکی, vol. 23, no. 123, pp. 31–42, 1395, [Online]. Available: https://sid.ir/paper/30394/en

Related Journal Papers