THE EFFECT OF WATER SOLUBLE CAROTENOIDS OF SAFFRON ON CELL-MEDIATED IMMUNITY IN BALB/C MICE

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RAJABIAN T.; GHAZANFARI T.; DANIALI F.

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Journal: DANESHVAR MEDICINE Year:2008 | Volume:15 | Issue:76 Page(s): 21-28

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Title

THE EFFECT OF WATER SOLUBLE CAROTENOIDS OF SAFFRON ON CELL-MEDIATED IMMUNITY IN BALB/C MICE

Author(s)

RAJABIAN T. | GHAZANFARI T. | DANIALI F. | Issue Writer Certificate

Keywords

SAFFRONQ2

CAROTENOIDSQ3

CELL MEDIATED IMMUNITYQ3

DTHQ4

CELL VIABILITYQ3

Abstract

Introduction: SAFFRON (thread-like reddish colored stigmas of Crocus sativus L. flowers) was used in traditional medicine for cancers and infectious diseases. Recently, anti-tumor, cytotoxic, anti-mutagenesis and antioxidant activities of SAFFRON metabolites, especially its CAROTENOIDS (crocins and crocetin), have been reported.Objective: Since the SAFFRON is used as a spice for food flavoring and coloring and it is also used as a traditional herbal drug, in this study the effect of SAFFRON water soluble CAROTENOIDS was assessed on cell mediated immune response in a BALB/c mice model.Materials & Methods: Crocus sativus L. stigmas were collected during flowering season and air dried at 25oC in the dark. First, dried stigmas (100 mg) were successively and exhaustively extracted with light petroleum and diethyl ether in a Soxhlet extractor in the dark to remove non-glycosidic CAROTENOIDS, lipids, picrocrocin and odor compounds. Then, for separation of water soluble CAROTENOIDS, stigma sample was extracted with distilled water in a 10-mL ground-glass stopper tube in a boiling water bath for 20 min. Then, the suspension was centrifuged at 3000 x g and at 10oC for 20 min to separate the vegetable tissue residue. The different doses of extract were injected to six groups of four BALB/c mice. The delayed-type hypersensivity (DTH) response and CELL VIABILITY (MTT test) were performed to determine CELL MEDIATED IMMUNITY.Results: Splenocytes that were extracted from treated mice with 10 or 20 mg/kg of water soluble CAROTENOIDS of SAFFRON have shown a significant (p<0.05) decrease in cellular viability at the absence of mitogene stimulation in vitro, and a significant decrease was observed in splenocytes cellular viability of mice that have been treated with 20 mg/kg of water soluble CAROTENOIDS in the presence of mitogene. In this study, all used doses did not have any significant effect on DTH response (p<0.05).Conclusion: Results of the present study showed that water soluble CAROTENOIDS of SAFFRON have a dose-dependent effect on cell mediated immune response. Further studies on other mechanisms such as cytokine patterns are needed for exact evaluation of immunomodulatory effects of SAFFRON CAROTENOIDS.

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APA: Copy

RAJABIAN, T., GHAZANFARI, T., & DANIALI, F.. (2008). THE EFFECT OF WATER SOLUBLE CAROTENOIDS OF SAFFRON ON CELL-MEDIATED IMMUNITY IN BALB/C MICE. DANESHVAR MEDICINE, 15(76), 21-28. SID. https://sid.ir/paper/31188/en

Vancouver: Copy

RAJABIAN T., GHAZANFARI T., DANIALI F.. THE EFFECT OF WATER SOLUBLE CAROTENOIDS OF SAFFRON ON CELL-MEDIATED IMMUNITY IN BALB/C MICE. DANESHVAR MEDICINE[Internet]. 2008;15(76):21-28. Available from: https://sid.ir/paper/31188/en

IEEE: Copy

T. RAJABIAN, T. GHAZANFARI, and F. DANIALI, “THE EFFECT OF WATER SOLUBLE CAROTENOIDS OF SAFFRON ON CELL-MEDIATED IMMUNITY IN BALB/C MICE,” DANESHVAR MEDICINE, vol. 15, no. 76, pp. 21–28, 2008, [Online]. Available: https://sid.ir/paper/31188/en

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