JUVENILE MYELOMONOCYTIC LEUKEMIA (JMML) CELLS SPONTANEOUSLY DIFFERENTIATE INTO DENDRITIC-CELL LIKE POPULATIONS IN VITRO

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SHAYAN P.

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Journal: Iranian Journal of Pediatrics Year:2008 | Volume:18 | Issue:3 Page(s): 213-221

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Title

JUVENILE MYELOMONOCYTIC LEUKEMIA (JMML) CELLS SPONTANEOUSLY DIFFERENTIATE INTO DENDRITIC-CELL LIKE POPULATIONS IN VITRO

Author(s)

SHAYAN P. | Issue Writer Certificate

Keywords

JUVENILE MYELOMONOCYTIC LEUKEMIAQ3

CD1AQ3

DENDRITIC CELLSQ3

SUSPENSION CELL CULTUREQ3

ELECTRON MICROSCOPYQ3

IMMUNOSTAININGQ3

Abstract

Objective: JUVENILE MYELOMONOCYTIC LEUKEMIA (JMML) is a rare myelodysplastic/ myeloproliferative malignancy of early childhood, characterized by monocytosis, hepatosplenomegaly and an aggressive clinical course.Methods: In semi-solid culture JMML progenitor cells proliferate spontaneously into colony forming units. In order to study the mechanisms of proliferation and differentiation of JMML cells we developed a suspension culture system without additional exogenous growth factor supplement. Mononuclear cells (MNC) from peripheral blood, bone marrow or spleen of 14 patients with JMML and 24 controls were studied.Findings: JMML cells expressed higher levels of the proliferation marker Ki67 (median 24% [7- 39%] vs a median of 3.5% in controls). 90% of JMML cells were CD68-positive (vs 35% in controls) and by day 7 all JMML samples contained CD1A- positive cells. ELECTRON MICROSCOPY demonstrated cytoplasmic vesicular structures resembling multilamellar MHC II comparetiments, which together with the expression of CD1A - support a dendritic cell (DC)-phenotype.Conclusion: Differentiation into CD1A-positive DC seems to be a frequent phenomenon in cultured JMML MNC, which in vivo may contribute to clinical characteristics such as skin and organ infiltration.

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APA: Copy

SHAYAN, P.. (2008). JUVENILE MYELOMONOCYTIC LEUKEMIA (JMML) CELLS SPONTANEOUSLY DIFFERENTIATE INTO DENDRITIC-CELL LIKE POPULATIONS IN VITRO. IRANIAN JOURNAL OF PEDIATRICS, 18(3), 213-221. SID. https://sid.ir/paper/76191/en

Vancouver: Copy

SHAYAN P.. JUVENILE MYELOMONOCYTIC LEUKEMIA (JMML) CELLS SPONTANEOUSLY DIFFERENTIATE INTO DENDRITIC-CELL LIKE POPULATIONS IN VITRO. IRANIAN JOURNAL OF PEDIATRICS[Internet]. 2008;18(3):213-221. Available from: https://sid.ir/paper/76191/en

IEEE: Copy

P. SHAYAN, “JUVENILE MYELOMONOCYTIC LEUKEMIA (JMML) CELLS SPONTANEOUSLY DIFFERENTIATE INTO DENDRITIC-CELL LIKE POPULATIONS IN VITRO,” IRANIAN JOURNAL OF PEDIATRICS, vol. 18, no. 3, pp. 213–221, 2008, [Online]. Available: https://sid.ir/paper/76191/en

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