PREPARATION OF T-2 TOXIN-PROTEIN CONJUGATES AND PRODUCTION OF ANTI T-2 ANTIBODY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AREFPOUR M.A.; SALEHI M.B.; SAJADI M.; SALIMIAN J.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: KOWSAR MEDICAL JOURNAL Year:2006 | Volume:10 | Issue:4 Page(s): 235-240

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,362

Download:

Cites:

Information Journal Paper

Title

PREPARATION OF T-2 TOXIN-PROTEIN CONJUGATES AND PRODUCTION OF ANTI T-2 ANTIBODY

Author(s)

AREFPOUR M.A. | SALEHI M.B. | SAJADI M. | SALIMIAN J. | Issue Writer Certificate

Keywords

T-2 TOXINQ4

KLHQ4

BSAQ4

CONJUGATIONQ4

ANTIBODYQ3

ELISAQ3

Abstract

Objective: To study different carrier's effect on ANTIBODY production against T-2 TOXIN. Materials and Methods: Three carrier BSA, KLH and poly lysine were utilized, after been succinated and then each was conjugated to the hydroxyl group of T-2 TOXIN. Ten Balb/c mice were initially injected with 20 micrograms of conjugate containing CFA (Complete Frunds Adjuvant). The mice received booster injections of 20 micrograms of conjugate containing IFA (Incomplete Frunds Adjuvant) every 3 weeks during and after which bleeding was done. The serum was removed and indirect ELISA was carried out. Results: The mice were immunized by BSA-T-2, but their serum recognized the BSA (carrier) much better than the T-2 (haptan). Conversely regarding KLH-T-2, T-2 (haptan) was recognized better than KLH, BSA and polylysine, by mice sera. Conclusion: This investigation indicates that KLH carrier is better than BSA for CONJUGATION because it an adjuvant for immune system and has very sites for CONJUGATION than BSA. Furthermore, KLH-T-2 conjugate elicits immune system response better and ANTIBODY titer was higher than BSA-T-2 conjugate.

Cites

No record.

References

No record.

Cite

APA: Copy

AREFPOUR, M.A., SALEHI, M.B., SAJADI, M., & SALIMIAN, J.. (2006). PREPARATION OF T-2 TOXIN-PROTEIN CONJUGATES AND PRODUCTION OF ANTI T-2 ANTIBODY. KOWSAR MEDICAL JOURNAL, 10(4), 235-240. SID. https://sid.ir/paper/32910/en

Vancouver: Copy

AREFPOUR M.A., SALEHI M.B., SAJADI M., SALIMIAN J.. PREPARATION OF T-2 TOXIN-PROTEIN CONJUGATES AND PRODUCTION OF ANTI T-2 ANTIBODY. KOWSAR MEDICAL JOURNAL[Internet]. 2006;10(4):235-240. Available from: https://sid.ir/paper/32910/en

IEEE: Copy

M.A. AREFPOUR, M.B. SALEHI, M. SAJADI, and J. SALIMIAN, “PREPARATION OF T-2 TOXIN-PROTEIN CONJUGATES AND PRODUCTION OF ANTI T-2 ANTIBODY,” KOWSAR MEDICAL JOURNAL, vol. 10, no. 4, pp. 235–240, 2006, [Online]. Available: https://sid.ir/paper/32910/en

Related Journal Papers