THE EFFECT OF Γ-INTERFERON ON NITRIC OXIDE RELEASE BY CYCLOPHOSPHAMIDE TREATED MACROPHAGES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AHMADI KAZEM; SOLGUI GH.; MOFEED M.; IMANI H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: KOWSAR MEDICAL JOURNAL Year:2005 | Volume:10 | Issue:2 Page(s): 111-120

Download Full-Text

Persian Verion

View:

1,171

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF Γ-INTERFERON ON NITRIC OXIDE RELEASE BY CYCLOPHOSPHAMIDE TREATED MACROPHAGES

Author(s)

AHMADI KAZEM | SOLGUI GH. | MOFEED M. | IMANI H. | Issue Writer Certificate

Keywords

CYCLOPHOSPHAMIDEQ2

LIPOPOLYSACHARIDEQ4

Γ-IFNQ3

NIITRIC OXIDEQ3

PRITONEAL MACROPHAGEQ4

ALVEOLAR MACROPHAGEQ4

Abstract

Objective: This project was designed to study the effect of γ-Interferon on NO release as an indicator of immune function involved in mice lung disease and resistance to opportunist infectious. Materials and Methods: Mice were subjected to interperitoneal injection of different concentrations of CYCLOPHOSPHAMIDE daily up to five days and left for 28 days. Mice were sacrificed and peritoneal cells were collected by interperitoneal injection of 5 ml cold PBS. The lavage was prepared by inject to and suction of 2ml cold PBS. After 3 times washings, peritoneal and alveolar cells were counted and resuspended in RPMI medium. Cells were incubated at 37°C and 5% CO2 in air for 2hs followed by three more washing to remove non-adherent cells. Macrophages were fed by 0.2 ml RPMI containing 10 µg/ml LPS and 100U γ -IFN. Cells were incubated at 37°C and 5% CO2 for 24h. Supernatants were collected and nitrite was measured by Griess method as an indicator of NO. Results: The results showed that CYCLOPHOSPHAMIDE at low concentration (1.5mg/day) reduced NO release by macrophages compared with the control group (48%±2, P<0.05). While ALVEOLAR MACROPHAGEs showed no significant differences in term of nitric oxide release. CYCLOPHOSPHAMIDE at high concentration (4.5mg/day) enhanced NO release by both peritopeal and ALVEOLAR MACROPHAGEs by 70% and 56% respectively. Treating the cells with combinations of LPS and γ -IFN enhanced NO production by 200% and 240% by peritoneal and ALVEOLAR MACROPHAGEs respectively. Conclution: It seems that part of immunosuppressive effect of CYCLOPHOSPHAMIDE might be due to reduction of NO release. Therefore, based on the positive effect of γ -IFN on NO release in groups which received high dosage of CYCLOPHOSPHAMIDE, it is supposed to use immune respose stimulators in groups under CYCLOPHOSPHAMIDE treatment may be warranted.

Cites

No record.

References

No record.

Cite

APA: Copy

AHMADI, KAZEM, SOLGUI, GH., MOFEED, M., & IMANI, H.. (2005). THE EFFECT OF Γ-INTERFERON ON NITRIC OXIDE RELEASE BY CYCLOPHOSPHAMIDE TREATED MACROPHAGES. KOWSAR MEDICAL JOURNAL, 10(2), 111-120. SID. https://sid.ir/paper/32959/en

Vancouver: Copy

AHMADI KAZEM, SOLGUI GH., MOFEED M., IMANI H.. THE EFFECT OF Γ-INTERFERON ON NITRIC OXIDE RELEASE BY CYCLOPHOSPHAMIDE TREATED MACROPHAGES. KOWSAR MEDICAL JOURNAL[Internet]. 2005;10(2):111-120. Available from: https://sid.ir/paper/32959/en

IEEE: Copy

KAZEM AHMADI, GH. SOLGUI, M. MOFEED, and H. IMANI, “THE EFFECT OF Γ-INTERFERON ON NITRIC OXIDE RELEASE BY CYCLOPHOSPHAMIDE TREATED MACROPHAGES,” KOWSAR MEDICAL JOURNAL, vol. 10, no. 2, pp. 111–120, 2005, [Online]. Available: https://sid.ir/paper/32959/en

Related Journal Papers