THE EFFECT OF PRE-EXPOSURE OF MICE PERITONEAL MACROPHAGES TO INTERFERON GAMMA IN TERM OF NITRIC OXIDE PRODUCTION

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AHMADI KAZEM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Studies in Medical Sciences Year:2001 | Volume:12 | Issue:1 Page(s): 56-64

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Title

THE EFFECT OF PRE-EXPOSURE OF MICE PERITONEAL MACROPHAGES TO INTERFERON GAMMA IN TERM OF NITRIC OXIDE PRODUCTION

Author(s)

AHMADI KAZEM | Issue Writer Certificate

Keywords

LIPOPOLYSACCHARIDE - G - IFN - NITRIC OXIDEQ3

MACROPHAGEQ3

Abstract

Introduction: It has previously been reported that MACROPHAGEs pre-exposed to interleukine-4 (a Th2 cytokine) no longer will respond to Lipopolysaccharide (LPS) in term of anti- Leishmanicidal activity and nitric oxide (NO) release. The aim of this study was to find out if pre-exposure of peritoneal MACROPHAGEs to g-IFN (a Th1 cytokine) could abolished the effect of LPS on MACROPHAGE in term of No release.Method & Material: Peritoneal cells were extracted in usual manner and plated out at 1×106 cells\well in 24 well plates with 2 ml RPMI-1640. After 2h incubation, non adherent cells were removed and MACROPHAGEs were incubated for further 24h in the presence or absence of LPS or pre-exposed to g-IFN before adding LPS.Results: The results showed that LPS enhanced NO release by peritoneal MACROPHAGEs in a dose dependent fashion.Discussion: Pre-exposure of MACROPHAGEs with g-IFN induced no further increase in NO production comparing with LPS-activated MACROPHAGEs.

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APA: Copy

AHMADI, KAZEM. (2001). THE EFFECT OF PRE-EXPOSURE OF MICE PERITONEAL MACROPHAGES TO INTERFERON GAMMA IN TERM OF NITRIC OXIDE PRODUCTION. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), 12(1), 56-64. SID. https://sid.ir/paper/64047/en

Vancouver: Copy

AHMADI KAZEM. THE EFFECT OF PRE-EXPOSURE OF MICE PERITONEAL MACROPHAGES TO INTERFERON GAMMA IN TERM OF NITRIC OXIDE PRODUCTION. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES)[Internet]. 2001;12(1):56-64. Available from: https://sid.ir/paper/64047/en

IEEE: Copy

KAZEM AHMADI, “THE EFFECT OF PRE-EXPOSURE OF MICE PERITONEAL MACROPHAGES TO INTERFERON GAMMA IN TERM OF NITRIC OXIDE PRODUCTION,” STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), vol. 12, no. 1, pp. 56–64, 2001, [Online]. Available: https://sid.ir/paper/64047/en

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