EVALUATION OF POSSIBLE OCCURRENCE OF MUTATION IN MMR REPAIR SYSTEM GENES IN RESISTANT AND SENSITIVECLINICAL STRAINS OF MYCOBACTERIUM TUBERCULOSISBY USING SEQUENCING METHOD

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AFZALI A.P.; ARJOMNDZADEGAN M.; AHMADI A.; HOSSEINI SH.; KAHBAZI M.; TOUSHEH M.

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Journal: IRANIAN SOUTH MEDICAL JOURNAL (ISMJ) Year:2016 | Volume:19 | Issue:3 Page(s): 351-360

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Title

EVALUATION OF POSSIBLE OCCURRENCE OF MUTATION IN MMR REPAIR SYSTEM GENES IN RESISTANT AND SENSITIVECLINICAL STRAINS OF MYCOBACTERIUM TUBERCULOSISBY USING SEQUENCING METHOD

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MYCOBACTERIUM TUBERCULOSISQ2

MUT T2Q1

PCRQ2

SEQUENCINGQ2

Abstract

Background: during recent years, the incidence and spread of drug resistance in MYCOBACTERIUM TUBERCULOSIS, the bacterium causing tuberculosis, has set this disease in World Health Organization priorities alignment of diseases like AIDS and hepatitis. Study of close examination of resistant and susceptible clinical strains genotypes is necessary to overcome drug resistance. Among the numerous repair systems, only there are limited number of encoding genes of DNA repair enzymes in MYCOBACTERIUM TUBERCULOSIS. Commonly these genes have been conserved and any changes among them likely increase the mutation occurance due to the impossibility of correction of spontaneous mutations insensitive strains of this bacteria. mut genes encode DNA repairable enzymes. This study investigated the mutations in these genes and the effect of these mutations on tuberculosis drug resistance.Materials& Methods: In this study, of 29 available specimens, we were selected 8 susceptible strains and 21 resistant strains and after ordering appropriate primers and performing the proliferation reaction two types of amp icons produced which including fragments of genes MUT T2 and mut T4 and they were sent in order to SEQUENCING.Results: The results of chain reaction primer represents an appropriate choice of primers which were investigated.Sequencing results showed that overall 73% of resistant strains that had been selected for study of mut T4 gene, have no mutations in codons 48of mutT4 gene, and 70% of resistant strains have no GGA>>>CGA mutation at codon 58 of MUT T2 gene.Conclusion: One of the strategies to overcome tuberculosis drug resistance is a close examination of genotypes of resistant and susceptible clinical strains. Results of this study was performed by examining changes in MUT T2 and mutT4 gene sequence. The mutation in MUT T2 always associated with mutation in mut T4, in this way, the first mutation may occurs inmut T4 and after that, the second mutation may occurs in MUT T2. Importance of this is determined by study of encoded proteins by these two genes and position of mut t4than MUT T2 in the MUT HLS spatial structure of protein complex. Results of comparison of drug resistance and occurrence of mutations in hotspots of MUT T2 and mut T4 genes illustrated that these genes are conserved in resistant strains. However, there is no significant relation in susceptible strains.

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APA: Copy

AFZALI, A.P., ARJOMNDZADEGAN, M., AHMADI, A., HOSSEINI, SH., KAHBAZI, M., & TOUSHEH, M.. (2016). EVALUATION OF POSSIBLE OCCURRENCE OF MUTATION IN MMR REPAIR SYSTEM GENES IN RESISTANT AND SENSITIVECLINICAL STRAINS OF MYCOBACTERIUM TUBERCULOSISBY USING SEQUENCING METHOD. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), 19(3 ), 351-360. SID. https://sid.ir/paper/33689/en

Vancouver: Copy

AFZALI A.P., ARJOMNDZADEGAN M., AHMADI A., HOSSEINI SH., KAHBAZI M., TOUSHEH M.. EVALUATION OF POSSIBLE OCCURRENCE OF MUTATION IN MMR REPAIR SYSTEM GENES IN RESISTANT AND SENSITIVECLINICAL STRAINS OF MYCOBACTERIUM TUBERCULOSISBY USING SEQUENCING METHOD. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ)[Internet]. 2016;19(3 ):351-360. Available from: https://sid.ir/paper/33689/en

IEEE: Copy

A.P. AFZALI, M. ARJOMNDZADEGAN, A. AHMADI, SH. HOSSEINI, M. KAHBAZI, and M. TOUSHEH, “EVALUATION OF POSSIBLE OCCURRENCE OF MUTATION IN MMR REPAIR SYSTEM GENES IN RESISTANT AND SENSITIVECLINICAL STRAINS OF MYCOBACTERIUM TUBERCULOSISBY USING SEQUENCING METHOD,” IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), vol. 19, no. 3 , pp. 351–360, 2016, [Online]. Available: https://sid.ir/paper/33689/en

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