مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

PREVALENCE OF BLACTX-M, BLASHV AND BLATEM β-LACTAMASE GENES AMONG ESCHERICHIA COLI ISOLATES IN FOODBORNE OUTBREAK IN IRAN

Pages

  48-52

Keywords

TEM 
SHV 
PCR 

Abstract

 Background: FExtended spectrum beta-lactamases (ESBLs) are known as enzymes that are responsible for creating high level of antimicrobial resistance in gram negative microorganisms especially in ESCHERICHIA COLI. Therefore, survey and identification of the genes generating these enzymes can be effective in controlling the resistance and helpful in using appropriate b-lactam drug against them.Objective: In this study we focused on evaluating the prevalence of ESBL enzyme genes, including CTX-M, SHV, and TEM b-lactamase, among E. coli isolates in foodborne disease outbreak.Materials and Methods: Four hundred stool samples from the individuals with diarrhea, nausea, vomiting, abdominal cramps, fever and headache were tested by IMVIC biochemical test for E. coli. ESBL genes were determined using antimicrobial susceptibility test based on the Clinical and Laboratory Standards Institute (CLSI), disk diffusion method, and confirmatory test (combined disk). Subsequently, polymerase chain reaction (PCR) was performed for following the relative genes.Results: The highest resistance in E. coli isolates was reported against ampicillin (56%), and 36% of samples were ESBL-positive. As determined by PCR, the prevalence of SHV, TEM, and CTX-M1 genes was 0%, 18%, and 38%, respectively.Conclusion: Due to the prevalence of b-lactamase genes, application of accurate and rapid identification methods such as PCR deems essential. Therefore, it can be considered as a routine method.

Cites

References

Cite

APA: Copy

SOLTAN DALLAL, MOHAMMAD MEHDI, Fani, Fereshteh, RAJABI, ZAHRA, KARAMI TALAB, MOHSEN, & MOLLA AGHA MIRZAEI, HEDROOSHA. (2018). PREVALENCE OF BLACTX-M, BLASHV AND BLATEM β-LACTAMASE GENES AMONG ESCHERICHIA COLI ISOLATES IN FOODBORNE OUTBREAK IN IRAN. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, 6(2), 48-52. SID. https://sid.ir/paper/347603/en

Vancouver: Copy

SOLTAN DALLAL MOHAMMAD MEHDI, Fani Fereshteh, RAJABI ZAHRA, KARAMI TALAB MOHSEN, MOLLA AGHA MIRZAEI HEDROOSHA. PREVALENCE OF BLACTX-M, BLASHV AND BLATEM β-LACTAMASE GENES AMONG ESCHERICHIA COLI ISOLATES IN FOODBORNE OUTBREAK IN IRAN. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN[Internet]. 2018;6(2):48-52. Available from: https://sid.ir/paper/347603/en

IEEE: Copy

MOHAMMAD MEHDI SOLTAN DALLAL, Fereshteh Fani, ZAHRA RAJABI, MOHSEN KARAMI TALAB, and HEDROOSHA MOLLA AGHA MIRZAEI, “PREVALENCE OF BLACTX-M, BLASHV AND BLATEM β-LACTAMASE GENES AMONG ESCHERICHIA COLI ISOLATES IN FOODBORNE OUTBREAK IN IRAN,” INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, vol. 6, no. 2, pp. 48–52, 2018, [Online]. Available: https://sid.ir/paper/347603/en

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