DISCRIMINATION BETWEEN NORMAL EXPRESSION AND OVEREXPRESSION OF MULTIDRUG RESISTANCE GENE (MDRI) BY RT-PCR METHOD

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NADALI FATEMEH; POURFATH ELAH A.A.; ALI MOGHADAM K.; DIZAJI A.; ZOMORODIPOUR A.; ROSTAMI SH.; AZIZI E.; GHAVAMZADEH ARDESHIR

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Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2004 | Volume:22 | Issue:73 Page(s): 23-26

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Title

DISCRIMINATION BETWEEN NORMAL EXPRESSION AND OVEREXPRESSION OF MULTIDRUG RESISTANCE GENE (MDRI) BY RT-PCR METHOD

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Abstract

Introduction. Resistance to chemotherapy is a major obstacle in cancer treatment. This phenomen is termed multidrug resistance (MDR). One of the mechanisems of MDR involves the overexpression of the multidrug resistance gene (MDR1) products, P- glycoprotein. The aim of this study was to identify the normal expression from overexpression of MDR1 gene by RT-PCR for detection of the MDR1 expression in clinical samples.Methods. In order to identify the normal expression from overexpression of MDR1 gene, we used the parental K562 cell line and its resistant subline to Doxorubicin by RT-PCR method. Also, we studied expression of MDR1 gene on peripheral blood leukocytes from 16 normal individuals, 5 cord blood samples and 5 samples of mononuclear cells from peripheral blood BMT donors on GM-CSF treatment.Results. The results showed MDR1 gene expression in normal individuals peripheral blood mononuclear cells and in parental K562 cell line by a 35 cycle of PCR but expression was disappeared by a 30 cycle of PCR. Also, in different subpopulation of normal leukocytes, in spit T cells, gene expression was not observed in granulocytes by a 30 cycles of PCR: MDR1gene expression was observed in mononuclear cells from cord blood and peripheral blood from donor on GM-CSF.Discussion. Because of the high sensitivity of RT-PCR method and normal expression of MDR1 gene in normal peripheral blood mononuclear cells, the RT-PCR alone is not sufficient for detection of multidrug resistance in hematopoietic malignancy and the cycle number of PCR should be adjusted for detection of MDR1 gene overexpression or it should be down by semi-quantitative or quantitative methods. Also, the normal elements of peripheral blood especially T cells should be eliminated from clinical samples before RT-PCR method.

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APA: Copy

NADALI, FATEMEH, POURFATH ELAH, A.A., ALI MOGHADAM, K., DIZAJI, A., ZOMORODIPOUR, A., ROSTAMI, SH., AZIZI, E., & GHAVAMZADEH, ARDESHIR. (2004). DISCRIMINATION BETWEEN NORMAL EXPRESSION AND OVEREXPRESSION OF MULTIDRUG RESISTANCE GENE (MDRI) BY RT-PCR METHOD. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 22(73), 23-26. SID. https://sid.ir/paper/358743/en

Vancouver: Copy

NADALI FATEMEH, POURFATH ELAH A.A., ALI MOGHADAM K., DIZAJI A., ZOMORODIPOUR A., ROSTAMI SH., AZIZI E., GHAVAMZADEH ARDESHIR. DISCRIMINATION BETWEEN NORMAL EXPRESSION AND OVEREXPRESSION OF MULTIDRUG RESISTANCE GENE (MDRI) BY RT-PCR METHOD. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2004;22(73):23-26. Available from: https://sid.ir/paper/358743/en

IEEE: Copy

FATEMEH NADALI, A.A. POURFATH ELAH, K. ALI MOGHADAM, A. DIZAJI, A. ZOMORODIPOUR, SH. ROSTAMI, E. AZIZI, and ARDESHIR GHAVAMZADEH, “DISCRIMINATION BETWEEN NORMAL EXPRESSION AND OVEREXPRESSION OF MULTIDRUG RESISTANCE GENE (MDRI) BY RT-PCR METHOD,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 22, no. 73, pp. 23–26, 2004, [Online]. Available: https://sid.ir/paper/358743/en

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