The Effects of Nicotine and Caffeine Treatment on the Vitality and Regenerative Potentials of Mesenchymal Stem Cells

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ZEINALI Z.; Abtahi Froushani M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF ADVANCED BIOMEDICAL SCIENCES Year:2019 | Volume:9 | Issue:2 Page(s): 1464-1473

Download Full-Text

Persian Verion

View:

643

Download:

Cites:

Information Journal Paper

Title

The Effects of Nicotine and Caffeine Treatment on the Vitality and Regenerative Potentials of Mesenchymal Stem Cells

Author(s)

ZEINALI Z. | Abtahi Froushani M. | Issue Writer Certificate

Keywords

Mesenchymal Stem cellQ1

CaffeineQ2

nicotineQ3

Abstract

Background & Objective: Previous studies indicated that Mesenchymal Stem cells (MSCs) express some of the nicotinic receptor subunits and adenosine receptors. Therefore, MSCs function may be controlled by two very consuming environmental substance like nicotine (nicotinic receptor-agonist) and Caffeine (adenosine antagonist). The purpose of the present study is to determine the role of nicotine or Caffeine on the some of the function of MSCs. Materials & methods: The Mesenchymal Stem cells were isolated from the bone marrow of NMRI. The cells were incubated with Caffeine (0. 1mM) and or with nicotine (0. 1μ M) for 48 hours. In a set of experiments, the cells were trypsinized and the vitality, the biological activity of cell membranes and the potentials mitochondrial redaction of them were assumed. In another experiments, the cutler plates were scratched with tip of the sampler, and the regeneration velocity of scar was evaluated by an optical camera. Results: Treatment of MSCs with nicotine caused a significant increase in the absolute number of MSCS, concurrent with an increase in mitochondrial function. While Caffeine decreased the vitality of cells, it also promoted a significant reduction in mitochondrial activity of remnant live cells. nicotine induced a significant reduction in membrane activity of cells, while Caffeine treatment exhibited a marked raise in neutral red uptake by MSCs compared to neutral red uptake by control cells. Regenerative assay showed that nicotine markedly promoted the regenerative potential of MSCs, while Caffeine markedly reversed the regenerative potential of MSCs. Conclusion: It seems that nicotine at used dose promoted the regenerative potential of MSCs, while Caffeine had a revers effects.

Cites

No record.

References

Cite

APA: Copy

ZEINALI, Z., & Abtahi Froushani, M.. (2019). The Effects of Nicotine and Caffeine Treatment on the Vitality and Regenerative Potentials of Mesenchymal Stem Cells. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 9(2 ), 1464-1473. SID. https://sid.ir/paper/367410/en

Vancouver: Copy

ZEINALI Z., Abtahi Froushani M.. The Effects of Nicotine and Caffeine Treatment on the Vitality and Regenerative Potentials of Mesenchymal Stem Cells. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2019;9(2 ):1464-1473. Available from: https://sid.ir/paper/367410/en

IEEE: Copy

Z. ZEINALI, and M. Abtahi Froushani, “The Effects of Nicotine and Caffeine Treatment on the Vitality and Regenerative Potentials of Mesenchymal Stem Cells,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 9, no. 2 , pp. 1464–1473, 2019, [Online]. Available: https://sid.ir/paper/367410/en

Related Journal Papers