Comparison of the effects of extremely low-frequency Electromagnetic field and Betaine on in vitro osteogenic differentiation of human adipose tissue derived-mesenchymal stem cells

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Tabatabai Tayebeh Sadat; HAJI GHASEM KASHANI MARYAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF TORBAT HEYDARIYEH UNIVERSITY OF MEDICAL SCIENCES Year:2020 | Volume:8 | Issue:3 Page(s): 23-35

Download Full-Text

Persian Verion

View:

394

Download:

Cites:

Information Journal Paper

Title

Comparison of the effects of extremely low-frequency Electromagnetic field and Betaine on in vitro osteogenic differentiation of human adipose tissue derived-mesenchymal stem cells

Author(s)

Tabatabai Tayebeh Sadat | HAJI GHASEM KASHANI MARYAM | Issue Writer Certificate

Keywords

cellsQ1

BetaineQ1

Electromagnetic fieldQ1

Osteogenic differentiationQ1

Abstract

Background & Aim: Extremely low-frequency Electromagnetic field (ELF-EMF) and Betaine are safe factors in bone fracture repair. This study aimed to compare the effects of these two stimuli on Osteogenic differentiation of human adipose stem cells (hADSCs). Methods: After obtaining written informed consent, cells were extracted from abdominal adipose tissue and then cultured in vitro until the third passage. Experimental groups were as follows: cells cultured in α-MEM and serum (control), in osteogenesis differentiation medium containing 10 mM Betaine (BET + OD), in α-MEM containing Betaine (BET), in OD and wave (50HZ and 1mT) (OD +EMF) and in the wave alone (EMF). Then, Osteogenic differentiation was assessed by morphology, evaluating the qualitative and quantitative Alizarin red staining, alkaline phosphatase activity and real time PCR of RUNX2 and OCN genes. Results: There was a significant increase in the formation of calcium deposits, alkaline phosphatase activity and also the expression of RUNX2 and OCN genes in OD + BET group compared to BET and OD + EMF groups. At the end of day 7, a significant decrease in alkaline phosphatase activity was observed in the EMF group compared to the OD + EMF and the BET groups. There was also a significant decrease in the expression of OCN gene in the EMF compared to the BET group. Conclusion: In this study, synergistic effect of Betaine and EMF on osteogenesis of hADSCs was demonstrated and this could be used for fracture repair and tissue engineering.

Cites

No record.

References

No record.

Cite

APA: Copy

Tabatabai, Tayebeh Sadat, & HAJI GHASEM KASHANI, MARYAM. (2020). Comparison of the effects of extremely low-frequency Electromagnetic field and Betaine on in vitro osteogenic differentiation of human adipose tissue derived-mesenchymal stem cells. JOURNAL OF TORBAT HEYDARIYEH UNIVERSITY OF MEDICAL SCIENCES, 8(3 ), 23-35. SID. https://sid.ir/paper/371199/en

Vancouver: Copy

Tabatabai Tayebeh Sadat, HAJI GHASEM KASHANI MARYAM. Comparison of the effects of extremely low-frequency Electromagnetic field and Betaine on in vitro osteogenic differentiation of human adipose tissue derived-mesenchymal stem cells. JOURNAL OF TORBAT HEYDARIYEH UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2020;8(3 ):23-35. Available from: https://sid.ir/paper/371199/en

IEEE: Copy

Tayebeh Sadat Tabatabai, and MARYAM HAJI GHASEM KASHANI, “Comparison of the effects of extremely low-frequency Electromagnetic field and Betaine on in vitro osteogenic differentiation of human adipose tissue derived-mesenchymal stem cells,” JOURNAL OF TORBAT HEYDARIYEH UNIVERSITY OF MEDICAL SCIENCES, vol. 8, no. 3 , pp. 23–35, 2020, [Online]. Available: https://sid.ir/paper/371199/en

Related Journal Papers