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Title

CLONINGVIRG GENE AND GENERATION MUTANT CONSTRUCT OF PGEM VIRG IN ORDER TO INDUCTION RECOMBINATION IN NATIVE SHIGELLA DYSENTERIAE

Pages

  184-190

Abstract

 Background: Shigellosis disease is the major causes of morbidity in children with diarrhea in Iran.ThevirG (icsA) gene plays a key role in pathogenesis and ability of invasion in shigella. The aim of this study was cloning, sequencingvirG gene and developing a mutant construct pGEMD virG in order to induction recombination in a native shigella for generation a LIVE ATTENUATED VACCINE CANDIDATE strain.Materials and methods: Initially, by use of biochemical tests, the native shigella strain was detected.ThevirG gene was cloned in pGEM-7zf vector and the nucleotide sequence was determined.According to the data of sequencing, digestion mapping of pGEMvirG vactor was obtained and a part ofvirG gene by using enzymatic digestion was removed. Finally, pGEM D virG construct was transformed toE. coli by utilization of chemical transformation method.Results: The native shigella strain by using biochemical tests was confirmed. The result of sequencing VIRG GENE (native strain) was submitted in NCBI Genebank database. The pGEMD virG construct contains a mutant construct ofvirG gene which 1751 bp was deleted through enzymatic digestion reaction and transformed inE. coli.Conclusion: Using the technique of ALLELIC EXCHANGE based on the incident of recombination in bacteria is one of the most effective methods to develop a disruption in the target genes. This mutant construct can be applied in development of a live attenuatedShigella dysenteriae vaccine candidate.

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    APA: Copy

    AHMADI DANESH, HORA, SAADATI, MOJTABA, DOROUDIAN, MOHAMMAD, YAKHCHALI, BAGHER, KARKHANEH, ALI ASGHAR, HOSSEINI, SAYED MOSTAFA, ZARE, MOKHTAR, & HASHEMI, MEHRAD. (2012). CLONINGVIRG GENE AND GENERATION MUTANT CONSTRUCT OF PGEM VIRG IN ORDER TO INDUCTION RECOMBINATION IN NATIVE SHIGELLA DYSENTERIAE. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 22(3 (69)), 184-190. SID. https://sid.ir/paper/98639/en

    Vancouver: Copy

    AHMADI DANESH HORA, SAADATI MOJTABA, DOROUDIAN MOHAMMAD, YAKHCHALI BAGHER, KARKHANEH ALI ASGHAR, HOSSEINI SAYED MOSTAFA, ZARE MOKHTAR, HASHEMI MEHRAD. CLONINGVIRG GENE AND GENERATION MUTANT CONSTRUCT OF PGEM VIRG IN ORDER TO INDUCTION RECOMBINATION IN NATIVE SHIGELLA DYSENTERIAE. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY[Internet]. 2012;22(3 (69)):184-190. Available from: https://sid.ir/paper/98639/en

    IEEE: Copy

    HORA AHMADI DANESH, MOJTABA SAADATI, MOHAMMAD DOROUDIAN, BAGHER YAKHCHALI, ALI ASGHAR KARKHANEH, SAYED MOSTAFA HOSSEINI, MOKHTAR ZARE, and MEHRAD HASHEMI, “CLONINGVIRG GENE AND GENERATION MUTANT CONSTRUCT OF PGEM VIRG IN ORDER TO INDUCTION RECOMBINATION IN NATIVE SHIGELLA DYSENTERIAE,” MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, vol. 22, no. 3 (69), pp. 184–190, 2012, [Online]. Available: https://sid.ir/paper/98639/en

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