CYTOKINESIS BLOCKED MICRONUCLEUS ASSAY FOR EVALUATION OF CHROMOSOMAL BREAKS IN ESOPHAGEAL CANCER

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

EMAMGHOLIZADEH MINAEI SORAYA; MOZDARANI HOSSEIN; AGHAMIRI SEYED MAHMOUD REZA; MOTAZAKKER MORTEZA; MANSOURI MOHSEN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Tehran University Medical Journal Year:2014 | Volume:72 | Issue:8 Page(s): 533-539

Download Full-Text

Persian Verion

View:

661

Download:

Cites:

Information Journal Paper

Title

CYTOKINESIS BLOCKED MICRONUCLEUS ASSAY FOR EVALUATION OF CHROMOSOMAL BREAKS IN ESOPHAGEAL CANCER

Author(s)

Keywords

ESOPHAGEAL NEOPLASMSQ2

LYMPHOCYTESQ2

MICRONUCLEIQ3

RADIOTHERAPYQ2

Abstract

Background: RADIOTHERAPY can cause DNA damage in normal cells, misrepaired or unrepaired double strand breaks in DNA lead to chromosomal breaks. As a result patient experience early and late effects in normal tissue during and after RADIOTHERAPY. Cytogenetic techniques can be used as a cancer predictive assay because there is an association between chromosome abnormalities and the risk of developing cancer. Also it can assess patient's complications during the therapy. The aim of the present study was evaluation of the cytogenetic alteration in peripheral blood LYMPHOCYTES of esophageal cancer patients treated with RADIOTHERAPY.Methods: The present study is an experimental and prospective research. It was done at RADIOTHERAPY department at Omid Center in Urmia from January to December 2012. Blood samples were obtained from 15 esophageal cancer patients, before (0 Gy), during (21.6 Gy), and after RADIOTHERAPY treatment (43.2 Gy). Blood samples were cultured in RPMI-1640 complete medium containing 1% phytohaemagglutinin and incubated in a CO2 incubator. Cytochalasin-B was added to the cultures at a final concentration of 5 mg/ml. Finally, harvesting, slide making, and analysis were performed according to standard procedures.Results: This study consisted of 15 patients, including 7 men and 8 women from 55 to 84 years old (70.07±11.548). Results indicate that, in the middle of treatment the average frequency of MICRONUCLEI increased significantly compared with their concurrent pre-treatment samples (greater than four-fold). Also, an increase in chromosome damage (MN frequency) proportional increasing radiation doses at the end of treatment was observed (P=0.001).Conclusion: Increasing in the MN frequency in the second and third stages is due to radiation effects. Thus, the use of the MN technique for assessing of the side effects in patients during the therapy is very helpful. Moreover, MN assay can be used as a predictive assay for detecting individuals (patient or healthy) with intrinsic radiosensitivity.

Cites

PRIMARY DNA DAMAGE IN TAXI DRIVERS WITH TRAFFIC RELATED AIR POLLUTION EXPOSURE: RESULTS OF COMET ASSAY TEST

References

No record.

Cite

APA: Copy

EMAMGHOLIZADEH MINAEI, SORAYA, MOZDARANI, HOSSEIN, AGHAMIRI, SEYED MAHMOUD REZA, MOTAZAKKER, MORTEZA, & MANSOURI, MOHSEN. (2014). CYTOKINESIS BLOCKED MICRONUCLEUS ASSAY FOR EVALUATION OF CHROMOSOMAL BREAKS IN ESOPHAGEAL CANCER. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 72(8), 533-539. SID. https://sid.ir/paper/39474/en

Vancouver: Copy

EMAMGHOLIZADEH MINAEI SORAYA, MOZDARANI HOSSEIN, AGHAMIRI SEYED MAHMOUD REZA, MOTAZAKKER MORTEZA, MANSOURI MOHSEN. CYTOKINESIS BLOCKED MICRONUCLEUS ASSAY FOR EVALUATION OF CHROMOSOMAL BREAKS IN ESOPHAGEAL CANCER. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2014;72(8):533-539. Available from: https://sid.ir/paper/39474/en

IEEE: Copy

SORAYA EMAMGHOLIZADEH MINAEI, HOSSEIN MOZDARANI, SEYED MAHMOUD REZA AGHAMIRI, MORTEZA MOTAZAKKER, and MOHSEN MANSOURI, “CYTOKINESIS BLOCKED MICRONUCLEUS ASSAY FOR EVALUATION OF CHROMOSOMAL BREAKS IN ESOPHAGEAL CANCER,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 72, no. 8, pp. 533–539, 2014, [Online]. Available: https://sid.ir/paper/39474/en

Related Journal Papers