P53 GENE MUTATIONS IN HODGKINS DISEASE

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SADRZADEH A.H.; JAHANZAD I.; KEYHANI M.; RAFIE R.; RAHMANIAN M.; HOSSEINI S.; MAHDIPOUR P.

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Paper Information

Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2005 | Volume:62 | Issue:12 Page(s): 1043-1048

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Title

P53 GENE MUTATIONS IN HODGKINS DISEASE

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Keywords

P53 GENE MUTATIONSQ4

HODGKINS DISEASEQ4

PCRQ4

SSCPQ4

MICRODISSECTIONQ4

Abstract

Background: P53 tumor-suppressor gene which encodes a nuclear phosphoprotein that arrests cell cycle progress at G1, is one of the most frequently mutated genes in human cancers.Materials and Methods: In this study, 40 cases of HODGKINS DISEASE (HD) which is the most common lymphoma in young adults, have been investigated for P53 GENE MUTATIONS. For each case, sections of paraffin-embedded tissue blocks were cut, a light hematoxylene stain was applied, and in order to enrich our samples for a higher percentage of Reed-Sternberg (RS) cells which are thought to be the neoplastic component of HD lesions, a MICRODISSECTION technique was used. We used polymerase chain reaction (PCR) amplitication technique and single strand conformational polymorphism analysis (SSCP) to study mutations within exons 5 to 8 of gene P53.Results: Six shifts were detected in five cases (12.5%), identified by SSCP (one shift in exon 5A, one in exon 5B, one in exon 6, two in exon 7 and one in exon 8). Conclusion: Since the specificity of PCR-SSCP is more than 95% for 100 to 300 bp polymerase chain reaction fragments, it is conceivable that P53 gene mutation is not a common feature in HD. However, the samples with abnormal SSCP require nucleotide sequencing studies to assess whether the abnormal migration represents a mutation or a polymorphism.

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APA: Copy

SADRZADEH, A.H., JAHANZAD, I., KEYHANI, M., RAFIE, R., RAHMANIAN, M., HOSSEINI, S., & MAHDIPOUR, P.. (2005). P53 GENE MUTATIONS IN HODGKINS DISEASE. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 62(12), 1043-1048. SID. https://sid.ir/paper/39540/en

Vancouver: Copy

SADRZADEH A.H., JAHANZAD I., KEYHANI M., RAFIE R., RAHMANIAN M., HOSSEINI S., MAHDIPOUR P.. P53 GENE MUTATIONS IN HODGKINS DISEASE. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2005;62(12):1043-1048. Available from: https://sid.ir/paper/39540/en

IEEE: Copy

A.H. SADRZADEH, I. JAHANZAD, M. KEYHANI, R. RAFIE, M. RAHMANIAN, S. HOSSEINI, and P. MAHDIPOUR, “P53 GENE MUTATIONS IN HODGKINS DISEASE,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 62, no. 12, pp. 1043–1048, 2005, [Online]. Available: https://sid.ir/paper/39540/en

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