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Information Journal Paper

Title

Design of DNA vaccine containing genes encoding sicl of Enterotoxigenic Escherichia coli (ETEC) and Enteromorrhagic Escherichia coli (EHEC)

Pages

  1-8

Abstract

 Introduction: Enterotoxigenic Escherichia coli (ETEC) and Enterohemorrhagic Escherichia coli O157: H7, the most common strains, are causes diarrhea that can kill hundreds of thousands of children annually. The development of an effective combination vaccine for Enterohemorrhagic Escherichia coli and Enterotoxigenic Escherichia coli is very important. Materials and Methods: In this study, the sicl gene was amplified and Subcloned as a DNA vaccine. The sequence of antigen encoding genes was evaluated from the genebank and their epitopes were evaluated to design of primer for the synthetic chimeric gene and was amplified by PCR. Subcloning of a multipartal chimeric gene in eukaryotic expression vector was performed to make a DNA vaccine and finally the protein was purified by nickel chromatography and evaluated by Western blotting. Results: The immunoblotting results of the expression of SICL Chimeric protein indicated the presence of a 76 kDa band in the form of insoluble particles after 12 hours induction. Purification of the recombinant protein using His tag sequencee and confirmation of the purified protein with the recombinant protein specific antibody demonstrated the accuracy of the protein expression. Discussion & Conclusion: Protein expression, purification and verify by western blotting showed that this recombinant Chimeric protein (SICL) can be expressed in eukaryotic host.

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    APA: Copy

    Jafari Parsa, Fatemeh, Zare, Shohreh, MIRHOSSEINI, SEYED ALI, & AMANI, JAFAR. (2020). Design of DNA vaccine containing genes encoding sicl of Enterotoxigenic Escherichia coli (ETEC) and Enteromorrhagic Escherichia coli (EHEC). PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 23(4 ), 1-8. SID. https://sid.ir/paper/412728/en

    Vancouver: Copy

    Jafari Parsa Fatemeh, Zare Shohreh, MIRHOSSEINI SEYED ALI, AMANI JAFAR. Design of DNA vaccine containing genes encoding sicl of Enterotoxigenic Escherichia coli (ETEC) and Enteromorrhagic Escherichia coli (EHEC). PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2020;23(4 ):1-8. Available from: https://sid.ir/paper/412728/en

    IEEE: Copy

    Fatemeh Jafari Parsa, Shohreh Zare, SEYED ALI MIRHOSSEINI, and JAFAR AMANI, “Design of DNA vaccine containing genes encoding sicl of Enterotoxigenic Escherichia coli (ETEC) and Enteromorrhagic Escherichia coli (EHEC),” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 23, no. 4 , pp. 1–8, 2020, [Online]. Available: https://sid.ir/paper/412728/en

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