DESIGNING A RECOMBINANT VACCINE CONTAINING THREE BACTERIAL PROTEINS OF EHEC, ETEC, AND SHIGELLA DYSENTERY ANTIGENS IN E. COLI AND EVALUATION OF ITS HUMORAL IMMUNITY IN MIC

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Ebadi Vahid; EBRAHIMI FIROUZ; HAJIZADEH ABBAS; Tarverdizadeh Yosuf; BAKHSHI MUSTAFA

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2018 | Volume:27 | Issue:157 Page(s): 1-16

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Title

DESIGNING A RECOMBINANT VACCINE CONTAINING THREE BACTERIAL PROTEINS OF EHEC, ETEC, AND SHIGELLA DYSENTERY ANTIGENS IN E. COLI AND EVALUATION OF ITS HUMORAL IMMUNITY IN MIC

Author(s)

Keywords

ENTEROBACTERIACEAEQ2

CHIMERIC ANTIGENQ1

DESIGNQ1

CHALLENGQ3

IMMUNIZATIONQ2

IN SILICO ANALYSISQ1

CANDIDATE VACCINEQ1

Abstract

Background and purpose: Different genera of ENTEROBACTERIACEAE have important roles ingastrointestinal diseases such as diarrhea. Among these, Enterotoxigenic E. coli (ETEC), enterohemorrhagic E. coli (EHEC), and Shigella are three main bacteria which are transmitted via contaminated food and water. Vaccination is a good strategy to combat these pathogens. In the present study, a CHIMERIC ANTIGEN, containingthe main antigens of these three bacteria was DESIGNed, expressed in E. coli host, and tested for protectionagainst EHEC strain. Materials and methods: Specific primers were DESIGNed to fuse these antigens. The propertiesof the CHIMERIC ANTIGEN, including physical and chemical properties, secondary and tertiary structures, andB cell and T cell epitopes were obtained via IN SILICO ANALYSIS. After the fusion of the genes, the resultingrecombinant pET28a(+) vector was transferred into the competent E. coli cells. Expression was inducedand the protein was purified. Then, the animals were immunized through subcutaneous administration ofthe antigen. Finally, the immunized animals were CHALLENGed by live E. coli O157: H7 cells. Results: IN SILICO ANALYSIS showed that the CHIMERIC ANTIGEN had appropriate physical andchemical properties which could be expressed in various expression systems. In addition, the antigen haspotent B-and T-cell epitopes. Experimental results showed that the antigen has efficiently provoked themice's humoral immune system. CHALLENGing the immunized mice by live bacterial cells showed that thechimeric antigen was able to protect the mice against E. coli O157: H7 strain. Conclusion: Both IN SILICO ANALYSIS and experimental results showed that the CHIMERIC ANTIGENhas appropriate properties which can be considered as a proper CANDIDATE VACCINE against the threebacteria mentioned.

Cites

Design of DNA vaccine containing genes encoding sicl of Enterotoxigenic Escherichia coli (ETEC) and Enteromorrhagic Escherichia coli (EHEC)

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APA: Copy

Ebadi, Vahid, EBRAHIMI, FIROUZ, HAJIZADEH, ABBAS, Tarverdizadeh, Yosuf, & BAKHSHI, MUSTAFA. (2018). DESIGNING A RECOMBINANT VACCINE CONTAINING THREE BACTERIAL PROTEINS OF EHEC, ETEC, AND SHIGELLA DYSENTERY ANTIGENS IN E. COLI AND EVALUATION OF ITS HUMORAL IMMUNITY IN MIC. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 27(157 ), 1-16. SID. https://sid.ir/paper/46022/en

Vancouver: Copy

Ebadi Vahid, EBRAHIMI FIROUZ, HAJIZADEH ABBAS, Tarverdizadeh Yosuf, BAKHSHI MUSTAFA. DESIGNING A RECOMBINANT VACCINE CONTAINING THREE BACTERIAL PROTEINS OF EHEC, ETEC, AND SHIGELLA DYSENTERY ANTIGENS IN E. COLI AND EVALUATION OF ITS HUMORAL IMMUNITY IN MIC. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;27(157 ):1-16. Available from: https://sid.ir/paper/46022/en

IEEE: Copy

Vahid Ebadi, FIROUZ EBRAHIMI, ABBAS HAJIZADEH, Yosuf Tarverdizadeh, and MUSTAFA BAKHSHI, “DESIGNING A RECOMBINANT VACCINE CONTAINING THREE BACTERIAL PROTEINS OF EHEC, ETEC, AND SHIGELLA DYSENTERY ANTIGENS IN E. COLI AND EVALUATION OF ITS HUMORAL IMMUNITY IN MIC,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 27, no. 157 , pp. 1–16, 2018, [Online]. Available: https://sid.ir/paper/46022/en

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